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Human TRAIL/TNFSF10 Alexa Fluor 488 MAb (Clone 75402) 100 TESTS

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Human TRAIL/TNFSF10 Alexa Fluor 488 MAb (Clone 75402) 100 TESTS信息二維碼

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產(chǎn)品介紹

    基本參數(shù)

    詳細說明

    • Species Reactivity

      Human

    • Specificity

      Detects human TRAIL in direct ELISAs.

    • Source

      Monoclonal Mouse IgG    1 Clone # 75402

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      Mouse myeloma cell line NS0-derived recombinant human TRAIL    
      Thr95-Gly281    
      Accession # P50591

    • Formulation

      Supplied in a saline solution containing BSA and Sodium Azide.

    • Label

      Alexa Fluor 488

    Applications


    • Recommended    
      Concentration

      Sample

    • Flow Cytometry

      5 μL/10    6 cells

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Flow Cytometry      
         

    Detection of TRAIL/TNFSF10 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 150 ng/mL IFN-alpha for 48 hours were stained with Mouse Anti-Human TRAIL/TNFSF10 Alexa Fluor? 488?conjugated Monoclonal Antibody (Catalog # FAB687G) and Mouse Anti-Human CD14 APC?conjugated Monoclonal Antibody (Catalog # ). Quadrant markers were set based on control antibody staining (Catalog # ). View our protocol for .

    Preparation and Storage

    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

    • Stability & Storage

      Protect from light.    Do not freeze.    

      • 12 months from date of receipt, 2 to 8 °C as supplied.

    Background: TRAIL/TNFSF10

    TRAIL is a type II transmembrane protein belonging to the TNF superfamily and is now designated TNFSF10. TRAIL is active as a homotrimer and is produced by a variety of cell types in both the membrane bound form and also as a soluble molecule. It binds to any of the four TRAIL receptors as well as to Osteoprotegerin.

    • Long Name:

      TNF-related Apoptosis-inducing Ligand

    • Entrez Gene IDs:

      8743 (Human); 22035 (Mouse)

    • Alternate Names:

      Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAIL; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10








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