• Species Reactivity

  Human

• Specificity

  Detects human ICAM?3/CD50 in ELISAs and Western blots. ICAM?3/CD50 was found not to recognize COS cells expressing the human ICAM?1 protein. The epitope for ICAM?3/CD50 has been found to be located on domain 1, or the interface between domains 1 and 2 of the human ICAM?3/CD50 molecule.

• Source

  Monoclonal Mouse IgG    ₁ Clone # ICAM-3.3

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Recombinant human ICAM-3 Fc Chimera

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  Recombinant Human ICAM?3/CD50 Fc Chimera (Catalog # )

• 
  

• Flow Cytometry

  2.5 μg/10    ⁶ cells

  Human peripheral blood monocytes

• 
  

• Immunoprecipitation

  Vazeux, R.     et al. (1992) Nature     360:485.

• 
  

• Immunocytochemistry

  8-25 μg/mL

• 
  

• Human ICAM-3/CD50 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 μg/mL 

  Human ICAM-3/CD50 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 μg/mL 

  Human ICAM?3/CD50 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human ICAM-3/CD50 Fc Chimera Protein, CF (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-3/CD50

Intercellular adhesion molecule 3 (ICAM-3), also known as CD50, is a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha  _d/beta  ₂. ICAM-3 is expressed on leukocytes and epidermal Langerhans cells.

• Long Name:

  Intercellular Adhesion Molecule 3

• Entrez Gene IDs:

  3385 (Human)

• Alternate Names:

  CD50 antigen; CD50; CDW50CDw50; ICAM3; ICAM-3; ICAM-Rintercellular adhesion molecule-3; intercellular adhesion molecule 3