• Species Reactivity

  Human, Mouse, Rat, Bovine, Canine, Primate

• Specificity

  Human, mouse, rat and bovine ~53 kDa GABA    _A R alpha 2 subunit

• Source

  Polyclonal Rabbit Serum

• Purification

  Antigen Affinity-purified

• Immunogen

  GABA    _A R alpha 2 subunit specific peptide

• Formulation

  50 μL unpurified neat rabbit serum.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1:1000 dilution

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R alpha 2

GABA  _A ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABA  _A receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABA  _A receptors have some combination of alpha, beta and gamma subunits. The rat alpha 2 isoform is a 53 kDa, 423 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 15 - 222). As with many receptors, phosphorylation is likely to be used as a regulatory mechanism. The alpha 2 subunit is known to be phosphorylated on Ser/Thr residues. Rat alpha 2 has multiple isoforms, all involving the 5′ UTR. These may dictate alpha 2 levels within the cell. This receptor subunit is particularly abundant in forebrain areas and is a potential target of anabolic steroids. Behavioral alterations associated with steroid abuse may be a manifestation of alpha 2 beta 3 gamma 2L receptor activation.

• References:

1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.

2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.

3. Khrestchatisky, M. et al. (1991) J. Neurochem. 56:1717.

4. Yang, P. et al. (2005) Neuropharmacology 49:300.

5. Tian, H. et al. (2005) Mol. Brain Res. 137:174.

6. Bureau, M.H. and J.J. Laschet (1995) J.Biol. Chem. 270:26482.

• Long Name:

  gamma-Aminobutyric Acid Type A Receptor, alpha-2 Polypeptide

• Entrez Gene IDs:

  2555 (Human); 14395 (Mouse); 289606 (Rat); 282236 (Bovine)

• Alternate Names:

  GABA A R alpha 2; GABA(A) receptor subunit alpha-2; GABAAR alpha 2; GABAARa2; GABRA2; gamma-aminobutyric acid (GABA) A receptor, alpha 2; gamma-aminobutyric acid receptor subunit alpha-2