• Species Reactivity

  Human

• Specificity

  Detects human Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 323519

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III    
  Gly34-Glu251    
  Accession # P13726

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 488

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is a 46 kDa integral membrane protein that is part of the extrinsic pathway of coagulation. It functions as a protein cofactor/receptor for Coagulation Factor VII, which is synthesized in the liver and circulates in the plasma (1). Upon binding to TF, the inactive Factor VII is rapidly converted into activated Factor VIIa. The resulting 1:1 complex of Factor VIIa and TF activates the coagulation pathway, and as a soluble form, plays an important coagulation-independent role in processes such as angiognesis (2-4). Synthesized as a 295 amino acid (aa) precursor, TF consists of a signal peptide (aa 1-32) and the mature chain (aa 33-295). The mature region contain an extracellular region (aa 33-251), followed by a transmembrane segment (aa 252-274) and a cytoplasmic tail (aa 275-295) (5-8). TF is found on endothelial cells, monocytes and vascular smooth muscle cells. Over aa 33-251, human and mouse TF share 58% aa sequence identity. 

• References:

1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659. 

2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. 

3. Gedding, J.E. and N. Mackman (2014) Thromb. Haemost. 111:570.

4. Hobbs, J.E. et al. (2007) Thromb. Res. 120 Suppl 2:S13.

5. Scarpati, E.M. et al. (1987) Biochemistry 26:5234.

6. Fisher, K.L. et al. (1987) Thromb. Res. 48:89.

7. Morrissey, J.H. et al. (1987) Cell 50:129.

8. Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.

• Entrez Gene IDs:

  2152 (Human); 14066 (Mouse)

• Alternate Names:

  CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor