• Assay Length

  3.5 hours

• Sample Type & Volume Required Per Well

  Cell Culture Supernates (51 uL), Serum (51 uL), EDTA Plasma (51 uL), Heparin Plasma (51 uL), Urine (51 uL)

• Sensitivity

  41.4 pg/mL

• Assay Range

  39.0 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

• Specificity

  Measures PGE    ₂ levels in various samples

• Cross-reactivity

  Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.

• Interference

  No significant interference observed with available related molecules.

Control Available  

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QC142, Parameter Immunoassay Control Set 777 Human Prostaglandin E2 -

Product Summary

The Parameter PGE  ₂ Immunoassay is a 3.5 hour forward sequential competitive enzyme immunoassay   designed to measure PGE₂ in cell culture supernates, serum, plasma, and urine.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
 

Recovery

The recovery of PGE₂ spiked to levels throughout the range of the assay in various matrices was evaluated.

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of PGE  ₂ were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Preparation and Storage

• Storage

  Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Prostaglandin E2/PGE2

Prostaglandin E2 (PGE2) is formed in a variety of cells from prostaglandin H2, which is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGE2 has a number of biological actions, including vasodilation, both anti- and proinflammatory action, modulation of sleep/wake cycles, and facilitation of human immunodeficiency virus replication. It elevates cAMP levels, stimulates bone resorption and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics.

• Alternate Names

  PGE2;