• Species Reactivity

  Mouse

• Specificity

  Detects mouse IL-22 R alpha 1 in direct ELISAs. In direct ELISAs, 25% cross-reactivity with recombinant human (rh) IL?22 R alpha 1 is observed and no cross-reactivity with rhIL-20 R alpha, recombinant mouse (rm) IL-20 R alpha, rhIL-22BP, or rmIL-22BP is observed.

• Source

  Monoclonal Rat IgG2a Clone # 496514

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse IL-22 R alpha 1    
  Thr18-Ala228    
  Accession # Q80XZ4

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 647

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  Hepa 1?6 mouse hepatoma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-22 R alpha 1

The IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa type I transmembrane glycoprotein that belongs to the type II cytokine receptor family (CRF). IL-22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 330 aa cytoplasmic domain (1). Within the ECD, mouse IL-22 R alpha 1 shares 78%, 78%, and 94% aa sequence identity with canine, human, and rat IL-22 R alpha 1, respectively. It shares 20%?26% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22, and -24 receptors (2). IL-22 R alpha 1/IL-10 R beta is an IL-22 responsive receptor (3, 4), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (5, 6). In both cases, IL?22 R alpha 1 functions as the high affinity ligand binding subunit, and subsequent association with IL-10 R beta or IL-20 R beta serves to stabilize the complex (3, 6?9). IL?22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but association with IL-10 R beta or IL-20 R beta is required for signal transduction (3, 7). IL-22BP functions as a competitive antagonist by binding IL-22 and preventing its association with IL-22 R alpha 1 (8, 10). Even though it is a receptor for interleukins, IL?22 R alpha 1 is not expressed on hematopoietic cells (7, 11, 12). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (7, 11?14). IL?22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (4, 12, 14, 15). IL?22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (4, 15).

• References:

1. Tachiiri, A. et al. (2003) Genes Immun. 4:153.

2. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. 

3. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335. 

4. Boniface, K. et al. (2005) J. Immunol. 174:3695. 

5. Dumoutier, L. et al. (2001) J. Immunol. 167:3545. 

6. Wang, M. et al. (2002) J. Biol. Chem. 277:7341. 

7. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725. 

8. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.

9. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099

10. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.

11. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.

12. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.

13. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.

14. Wolk, K. et al. (2004) Immunity 21:241.

15. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.

• Long Name:

  Interleukin 22 Receptor

• Entrez Gene IDs:

  58985 (Human); 230828 (Mouse)

• Alternate Names:

  CRF2-9; CRF2-9interleukin 22 receptor; Cytokine receptor class-II member 9; Cytokine receptor family 2 member 9; IL-22 R alpha 1; IL-22 receptor subunit alpha-1; IL22R alpha 1; IL22R; IL22R1; IL22RA1; IL-22Ra1; IL-22R-alpha-1; IL-TIF-R1; interleukin 22 receptor, alpha 1; interleukin-22 receptor subunit alpha-1; zcytoR11