• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.1 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of Caki?2 human clear cell carcinoma epithelial cells. When 3 x 10    ⁴ cells/well are added to rhDesmoglein-3 coated plates (10 μg/mL, 100 μL/well), approximately 45?65% will adhere after 90 minutes at 37° C.

• Source

  Mouse myeloma cell line, NS0-derived

  Human Desmoglein-3 (Glu50-Arg615) Accession # P32926	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Glu50

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  89.5 kDa (monomer)

• SDS-PAGE

  105-110 kDa, reducing conditions

Background: Desmoglein-3

Desmoglein-3, also known as pemphigus vulgaris antigen (PVA), is a 130 kDa transmembrane glycoprotein that belongs to the cadherin family of calcium dependent adhesion molecules (1 - 3). Human Desmoglein-3 is synthesized with a 26 amino acid (aa) propeptide. The mature protein consists of a 566 aa extracellular domain (ECD) that contains four cadherin-like domains, a 25 aa transmembrane segment, and a 359 aa cytoplasmic domain (4). Within the ECD, human Desmoglein-3 shares 78% aa sequence identity with mouse and rat Desmoglein-3. It shares 44%, 47%, and 56% aa sequence identity with Desmoglein-1, -2, and -4 respectively. Desmoglein-3 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (4 - 6). During apoptosis, Desmoglein-3 is cleaved by caspases, plus MMP-2, and MMP-9 at sites within the cytoplasmic and extracellular regions, resulting in shortened transmembrane forms and a soluble 75 kDa ECD fragment (7, 8). The downregulation of Desmoglein-3 in oral squamous cell carcinoma correlates with metastatic potential (9). Desmoglein-3 is the target of autoantibodies in pemphigus vulgaris, a blistering skin disorder with compromised inter-keratinocyte adhesion (6, 10). Binding of these antibodies triggers Desmoglein-3 internalization and degradation by keratinocytes (10).

• References:

1. Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.

2. Garrod, D.R. et al. (2002) Mol. Membr. Biol. 19:81.

3. Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.

4. Amagai, M. et al. (1991) Cell 67:869.

5. Silos, S.A. et al. (1996) J. Biol. Chem. 271:17504.

6. Amagai, M. et al (1996) J. Invest. Dermatol. 106:351.

7. Weiske, J. et al. (2001) J. Biol. Chem. 276:41175.

8. Cirillo, N. et al. (2007) Oral Dis. 13:341.

9. Wang, L. et al. (2007) Virchows Arch. Sept. 11 epub.

10. Calkins, C.C. et al. (2006) J. Biol. Chem. 281:7623.

• Entrez Gene IDs:

  1830 (Human)

• Alternate Names:

  Cadherin family member 6; CDHF6; CDHF6130-kD pemphigus vulgaris antigen; desmoglein 3 (pemphigus vulgaris antigen); desmoglein 3; Desmoglein3; Desmoglein-3; DKFZp686P23184,130 kDa pemphigus vulgaris antigen; DSG3; pemphigus vulgaris antigen; PVA