• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 10    ⁴ cells/well are added to Recombinant Human ICAM-5/Fc Chimera coated plates (25 μg/mL, 100 μL/well), >25% will adhere after 1 hour incubation at 37 °C.    
     Optimal dilutions should be determined by each laboratory for each application.  

• Source

  Mouse myeloma cell line, NS0-derived

  Human ICAM-5 (Ala28-Glu570) Accession # Q9UMF0	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Ala28

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  85.1 kDa (monomer)

• SDS-PAGE

  125-130 kDa, reducing conditions

Background: ICAM-5

Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is a cell surface glycoprotein belonging to the immunoglobulin superfamily. Human ICAM-5 consists of an 832 amino acid (aa) extracellular domain containing 9 immunoglobulin (Ig) domains and 15 N-glycosylation sites, a 28 aa transmembrane domain, and a 64 aa cytoplasmic domain. ICAM-5 shares 38 - 55% aa identity with other ICAMs, being most closely related to ICAM-1 (50% identity) and ICAM-3 (55% identity) (1). Human and mouse ICAM-5 share 85% aa identity.

The tissue distribution of ICAM-5 is unique among ICAMs, being expressed only in telencephalic regions of the central nervous system (2). Like other ICAMs, ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) (3). Binding of ICAM-5 to LFA-1 is dependent on the first amino terminal Ig domain of ICAM-5 (4). ICAM-5 also displays homophilic binding, with the amino terminal Ig domain binding to Ig domains 4 - 5. Homophilic binding of ICAM-5 is dependent of ICAM-5 being in a monomeric form. The monomeric form of ICAM-5 is found during dendritogenesis in developing brain, whereas a high molecular weight complex is found in mature neurons (5).

• References:

1. Mizuno, T. et al. (1997) J. Biol. Chem. 272:1156.

2. Yoshihara, Y. et al. (1994) Neuron 12:541.

3. Tain, L. et al. (1997) J. Immunol. 158:928.

4. Tain, L. et al. (2000) Eur. J. Immunol. 30:810.

5. Tain, L. et al. (2000) J. Immunol. 150:243.

• Long Name:

  Intercellular Adhesion Molecule 5

• Entrez Gene IDs:

  7087 (Human); 15898 (Mouse)

• Alternate Names:

  ICAM5; ICAM-5; intercellular adhesion molecule 5; intercellular adhesion molecule 5, telencephalin; Telencephalin; TLCNTelencephalin; TLNtelencephalin