• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of Y?79 human retinoblastoma cells. The ED    ₅₀ for this effect is 0.8-4 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived

  Human NrCAM Leu30-Asn600 (Ala526Pro) Accession # Q14CA1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Leu30

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  90 kDa (monomer)

• SDS-PAGE

  120-130 kDa, reducing conditions

Background: NrCAM

NrCAM, also known as Bravo, belongs to the L1 family of cell adhesion molecules which also includes L1CAM, Neurofascin, and CHL-1/L1CAM-2 (1). These molecules are type I transmembrane proteins that have 6 Ig-like domains and 4-5 fibronectin type III-like domains in their extracellular domain. L1 family cell adhesion molecules are expressed primarily in the nervous system where they share overlapping functions in controlling axonal growth and guidance (2). Mature human NrCAM is an approximately 200 kDa molecule that consists of a 1143 amino acid (aa) extracellular domain (ECD) with 6 Ig-like domains followed by 5 fibronectin type III domains; a 23 aa transmembrane segment, and a 114 aa cytoplasmic domain (3). Within the region of Ig-like domains, human NrCAM shares 92% aa sequence identity with mouse and rat NrCAM. Alternative splicing generates additional isoforms with deletions in the juxtamembrane region of the ECD plus short deletions near the N-terminus, between the 2  ^nd and 3  ^rd Ig-like domains, or following the 6  ^th Ig-like domain. A 140 kDa soluble fragment of the ECD can be released by proteolytic cleavage (4, 5). NrCAM is expressed on cerebellar granule neurons, retinal ganglion cells (RGC), star pyramidal cells in visual cortex, thalamocortical axons, and glial cells (4, 6-11). It is found on both axons and dendritic spines (7, 9). NrCAM mediates homophilic adhesion as well as heterophilic adhesion with Contactin, Contactin-2/TAG1, Neurofascin, PTP beta zeta, and Integrin  alpha 4 beta 1 (5, 12-15) and also interacts with Neuropilin-2, Plexin A3, and EphB2 (8-10). Depending on its interacting partners, NrCAM can promote or inhibit axon and neurite extension (6, 7, 11, 15) and mediate Semaphorin 3F induced neuronal growth cone collapse (9, 10). NrCAM plays an important role in the development of normal vision by regulating RGC axon pathfinding and mapping to the visual cortex (7, 8, 10). It is up-regulated in papillary thyroid carcinomas and the shed form can promote tumorigenesis (5, 16).

• References:

1. Sakurai, T. (2012) Mol. Cell. Neurosci. 49:351.

2. Stoeckli, E.T. and L.T. Landmesser (1995) Neruon 14:1165.

3. Lane, R.P. et al. (1996) Genomics 35:456.

4. Sakurai, T. et al. (2001) J. Cell Bio. 154:1259.

5. Conacci-Sorrell, M. et al. (2005) Cancer Res. 65:11605.

6. Faivre-Sarrailh, C. et al. (1999) J. Cell Sci. 112:3015.

7. Zelina, P. et al. (2005) Development 132:3609.

8. Dai, J. et al. (2013) PLoS One 8:e73000.

9. Demyanenko, G.P. et al. (2014) J. Neurosci. 34:11274.

10. Demyanenko, G.P. et al. (2011) J. Neurosci. 31:1545.

11. Feinberg, K. et al. (2010) Neuron 65:490.

12. Mauro, V.P. et al. (1992) J. Cell Biol. 119:191.

13. Sakurai, T. et al. (1997) J. Cell Biol. 136:907.

14. Lustig, M. et al. (1999) Dev. Biol. 209:340.

15. Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.

16. Gorka, B. et al. (2007) Br. J. Cancer 97:531.

• Long Name:

  Neuronal Cell Adhesion Molecule

• Entrez Gene IDs:

  4897 (Human); 319504 (Mouse)

• Alternate Names:

  Bravo; hBravo; KIAA0343Neuronal surface protein Bravo; MGC138845; MGC138846; neuronal cell adhesion molecule; NgCAM-related cell adhesion molecule; ng-CAM-related; NrCAM; nr-CAM