• Purity

  >95%, by SDS-PAGE with silver staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured in a cell proliferation assay using RT4?D6P2T rat schwannoma cells. The ED    ₅₀ for this effect is 0.15-0.75 μg/mL.

• Source

  E. coli-derived Gln23-Asn166, with an N-terminal Met

• Accession #

• N-terminal Sequence    
  Analysis

  Met

• Predicted Molecular Mass

  16 kDa

• SDS-PAGE

  16 kDa, reducing conditions

Data Images

Background: Reg1B

Reg1B also called Lithostatine-1-beta or Pancreatic stone protein 2 (PSP2) is a type I subclass member of the Reg gene family. The Reg multigene family consists of four subclasses, types I, II, III and IV based on the primary structures of the encoded proteins of the genes. Reg1B is also a member of the Reg family of secreted C-type lectin domain-containing pancreatic proteins (1, 2). The human Reg1B cDNA encodes 166 amino acids protein which contains a 22 aa signal peptide and the 144 aa secreted mature protein. Reg1B shares 86% animo acide sequence identity with Reg1A and like Reg1A is secreted by the exocrine pancreas and associated with islet cell regeneration and diabetogenesis. Reg family proteins were also shown to cause Schwann cell proliferation during regeneration of motor neurons (3).

• References:

1. Liu, J.-L. et al. (2008) Endocrine Metab. Immune Disord. Drug Targets 8:1.

2. Unno, M. et al. (1993) J. Biol. Chem. 268:15974.

3. Livesey F.J. et al. (1990) Nature. 390:614.

• Long Name:

  Regenerating Islet-derived 1B

• Entrez Gene IDs:

  5968 (Human)

• Alternate Names:

  Lithostathine 1 beta; lithostathine-1-beta; Pancreatic stone protein 2; pancreatic threadprotein); PSPS2; PSPS2PSP-2; Reg1B; regenerating islet-derived 1 beta; Regenerating islet-derived protein 1-beta; Regenerating protein I beta; REGH; REGLREG-1-beta; secretory pancreatic stone protein 2