• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <1.0 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When rhBMP-4 is coated at 1 μg/mL, rhRGM-A binds with an apparent K    _D <50nM. Immobilized rhRGM-A can bind rmNeogenin/Fc Chimera with a linear range of    
  10-500 ng/mL.

• Source

  Mouse myeloma cell line, NS0-derived Cys48-Gly422 & Pro169-Gly422, both with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Cys48 & Pro169    
   

• Predicted Molecular Mass

  42.4 kDa

• SDS-PAGE

  Multiple bands between 25-37 kDa, reducing conditions

Background: RGM-A

Human Repulsive Guidance molecule (RGM) is a 33 kDa GPI-linked member of an expanding RGM-related family of neuronal and muscle-expressed membrane proteins (1). It is synthesized as a 450 amino acid (aa) preproprotein that contains a 47 aa signal sequence, a 121 aa N-terminal prosegment, a 256 mature region and a 26 aa C-terminal prosegment (2). The N-terminal prosegment contains an RGD tripeptide and the molecule’s only two potential N-linked glycosylation sites. The mature segment shows an abbreviated von Willebrand factor domain. Proteolytic processing occurs at an aspartic acid-proline bond, creating a predicted 32 kDa mature region (2). The mature region of human RGM-A has 88% and 93% aa identity to the chick and mouse mature region of RGM-A, respectively. When compared to human RGMb and c, the mature region of human RGM-A shows 58% and 54% aa identity, respectively. Recombinant chick RGM-A has been reported to induce collapse of temporal but not nasal growth cones, and to repel temporal retinal axons in vitro. This suggests a role in the development of the retina-superior colliculus connection. In mammals, however, this activity is not so evident, and thus its function in this system is uncertain (3). Alternatively, in mice, RGM-A is said to be needed for neural tube closure, and may play a role in entorhinal-hippocampal connections (3, 4). The receptor for RGM-A is reported to be neogenin (5, 6). RGM-A has also been shown to be a bone morphogenic protein co-receptor, able to bind both BMP-2 and BMP-4 (7).

• References:

1. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.

2. Monnier P. et al. (2002) Nature 419:392.

3. Niederkofler V. et al. (2004) J. Neurosci. 24:808.

4. Brinks, H. et al. (2004) J. Neurosci. 24:3862.

5. Rajagopalan S. et al. (2004) Nat. Cell Biol. 6:756.

6. Matsunaga E. et al. (2004) Nat. Cell Biol. 6:749.

7. Babitt J.L. et al. (2005) J. Biol. Chem. 280(33):29820.

• Long Name:

  Repulsive Guidance Molecule A

• Entrez Gene IDs:

  56963 (Human); 244058 (Mouse)

• Alternate Names:

  RGM domain family member A; RGM domain family, member A; RGMA; RGM-A; RGMrepulsive guidance molecule A