• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to induce ERK phosphorylation in BaF3 mouse pro?B cells transfected with human ESAM. Stimulation of 2.5 x 10    ⁵ serum-starved hESAM transfected BaF3 cells with 25 μg Recombinant Human ESAM Fc Chimera (Catalog # ) for 30 minutes at 37° C leads to ERK phosphorylation.

• Source

  Mouse myeloma cell line, NS0-derived

  Human ESAM (Gln30 - Ala247) Accession # Q96AP7	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained: Gln30 predicted

• Predicted Molecular Mass

  50.3 kDa (monomer)

• SDS-PAGE

  67-75 kDa, reducing conditions

Background: ESAM

Endothelial cell-selective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules (1, 2). Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V-type and one C2-type Ig domain and is involved in homophilic adhesion (1). In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI-1 (3). The extracellular region of human ESAM shows 90%, 74%, 69% and 67% aa identity with monkey, canine, mouse and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes, and can be found associated with cell-to-cell junctions. Whether ESAM is restricted to a particular junctional type is not clear (1, 2). ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF-2 (4).

• References:

1. Hirata, K-I, et al. (2001) J. Biol. Chem. 276:16223.

2. Nasdala, I. et al. (2002) J. Biol. Chem. 277:16294.

3. Wegmann, F. et al. (2004) Exp. Cell Res. 300:121.

4. Ishida, T. et. al. (2003) J. Biol. Chem. 278:34598.

• Long Name:

  Endothelial Cell Adhesion Molecule

• Entrez Gene IDs:

  90952 (Human); 69524 (Mouse)

• Alternate Names:

  2310008D05Rik; endothelial cell adhesion molecule; endothelial cell-selective adhesion molecule; ESAM; HUEL (C4orf1)-interacting protein; LP4791 protein; W117m