• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to enhance the adhesion of Saos?2 human osteosarcoma cells to human Fibronectin.

  When 5 x 10⁴ cells/well are added to human Syndecan-1 and human Fibronectin (0.5 μg/mL, Catalog # 1918-FN) coated plates, cell adhesion is enhanced in a dose dependent manner. The ED₅₀ for this effect is 2.5?10 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived Gln18-Glu251, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained: Gln18 predicted

• Predicted Molecular Mass

  24.9 kDa

• SDS-PAGE

  65-90 kDa, reducing conditions

Background: Syndecan-1/CD138

Syndecan-1, designated CD138, is a dimeric type I transmembrane (TM) protein that belongs to the syndecan family of Type 1 transmembrane proteins (1, 2). The four syndecan family members are major carriers of heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans (GAGs) that have different expression patterns and extracellular sequences. Syndecan-1 forms weak non-covalent homodimers, or heterodimers with Syndecan-2 or -3, through interactions of the transmembrane domain (3). It is synthesized as a 310 amino acid (aa) precursor with a 17 aa signal sequence, a 234 aa extracellular domain (ECD) that includes three closely-spaced consensus Ser-Gly HS attachment sites near the N-terminus, a 25 aa TM segment, and a 34 aa cytoplasmic region that includes a PDZ binding motif with a tyrosine phosphorylation site. The ECD is variably modified by GAGs, producing molecular weights of 120 - 200 kDa for native Syndecan-1. Soluble forms are shed via proteolytic cleavage. Human Syndecan-1 ECD shares 65 - 71% aa identity with the ECD of rat, mouse, canine, equine and bovine Syndecan-1. Syndecan-1 shows highest expression on epithelial cells such as keratinocytes, and terminally differentiated B cells such as plasma cells (4, 5). It aids wound healing in skin, cornea, and heart following myocardial infarction by promoting re-epithelialization, migration, and collagen deposition (4 - 8). It binds chemokines, creating chemotactic gradients when shed, but also binds and modulates integrins to control the influx of leukocytes (5, 7, 9). The net effect is to allow, but limit, inflammation. In myeloma and other cancers, shedding of Syndecan-1 can facilitate growth, angiogenesis and metastasis (10 - 12). Growth factors, such as FGFs and HGF, bind GAG chains and use Syndecan-1 as a coreceptor (12, 13). The GAG chains may also be used by a variety of viruses and bacteria for cell adhesion and uptake (4).

• References:

1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.

2. Mali, M. et al. (1990) J. Biol. Chem. 265:6884.

3. Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.

4. Fears, C.Y. and A. Woods (2006) Matrix Biol. 25:443.

5. Stepp, M.A. et al. (2002) J. Cell Sci. 115:4517.

6. Ojeh, N. et al. (2008) J. Invest. Dermatol. 128:26.

7. Stepp, M.A. et al. (2007) J. Cell Sci. 120:2851.

8. Vanhoutte, D. et al. (2007) Circulation 115:475.

9. Li, Q. et al. (2002) Cell 111:635.

10. Beauvais, D.M. et al. (2009) J. Exp. Med. 206:691.

11. Yang, Y. et al. (2007) J. Biol. Chem. 282:13326.

12. Derksen, P.W.B. et al. (2002) Blood 99:1405.

13. Su, G. et al. (2007) J. Biol. Chem. 282:14906.

• Entrez Gene IDs:

  6382 (Human); 20969 (Mouse); 100338470 (Rabbit)

• Alternate Names:

  CD138 antigen; CD138; SDC; SDC1; SYND1heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan 1; syndecan proteoglycan 1; syndecan; Syndecan1; Syndecan-1