• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of Neuro?2A mouse neuroblastoma cells. When 5 x 10    ⁴ cells/well are added to rhFLRT1 coated plates (2.5 μg/mL, 100 μL/well), approximately 50%-70% will adhere after 30 minutes at 37° C.    
     Optimal dilutions should be determined by each laboratory for each application.  

• Source

  Mouse myeloma cell line, NS0-derived Ile21-Pro524, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Ile21

• Predicted Molecular Mass

  56.3 kDa

• SDS-PAGE

  70-80 kDa, reducing conditions

Background: FLRT1

FLRT1 is one of three FLRT (fibronectin, leucine rich repeat, transmembrane) glycoproteins expressed in distinct areas of the developing brain and other tissues (1, 2). The 90 kDa type I transmembrane (TM) human FLRT1 is synthesized as a 646 amino acid (aa) precursor with a 20 aa signal sequence, a 504 aa extracellular domain (ECD), a 21 aa TM segment and a 101 aa cytoplasmic region. The ECD contains 10 N-terminal leucine-rich repeats flanked by cysteine-rich areas, and a juxtamembrane fibronectin type III domain (1). The human FLRT1 ECD shares 97% aa sequence identity with mouse, rat, and canine, and 95% with bovine FLRT1 ECD. Human FLRT2 and FLRT3 ECDs share 47% and 61% aa identity, respectively, with FLRT1. The fibronectin domain of all three FLRTs can bind FGF receptors (2). This binding is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization of FLRTs in areas of cell contact and homotypic cell-cell association (4). This may be through direct interactions with other FLRT molecules or, as has been shown for FLRT3, by regulating internalization of adhesion molecules such as cadherins (4, 5). Both during development and in adulthood, FLRT1 mRNA is predominantly expressed in brain and kidney (1). FLRT1 is expressed at brain compartmental boundaries in mouse embryos (2). Its expression is distinct from that of FLRT2 and FLRT3 (2).

• References:

1. Lacy, S. E. et al. (1999) Genomics 62:417.

2. Haines, B. P. et al. (2006) Dev. Biol. 297:14.

3. Bottcher, R. T. et al. (2004) Nat. Cell Biol. 6:38.

4. Karaulanov, E. E. et al. (2006) EMBO Rep. 7:283.

5. Ogata, S. et al. (2007) Genes Dev. 21:1817.

• Long Name:

  Fibronectin Leucine Rich Transmembrane Protein 1

• Entrez Gene IDs:

  23769 (Human)

• Alternate Names:

  fibronectin leucine rich transmembrane protein 1; Fibronectin-like domain-containing leucine-rich transmembrane protein 1; FLRT1; leucine-rich repeat transmembrane protein FLRT1; MGC21624