• Purity

  >95%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of BUD?8 human fibroblast cells. The ED    ₅₀ for this effect is 1.5-7.5 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived Lys33-Ser561, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Lys33

• Predicted Molecular Mass

  52 kDa

• SDS-PAGE

  60-75 kDa, reducing conditions

Background: Corneodesmosin

Corneodesmosin, also known as CDSN and the S gene product, is a highly polymorphic secreted glycoprotein that plays an important structural role in the skin (1). Mature mouse Corneodesmosin shares 62% and 82% amino acid sequence identity with human and rat Corneodesmosin, respectively. It is expressed by differentiated keratinocytes in the corneal layer of the skin and is a major component of corneodesmosomes (2-4). It is also expressed in the inner root sheath of hair follicles (5, 6). Corneodesmosome has a high content of glycine, serine, and proline residues that promote its folding into a series of Gly-loop domains (2, 7). Corneodesmosin forms oligomers and associates homophilically to strengthen the adhesion between corneocytes (8, 9). Corneodesmosin-deficient mice exhibit a detachment of the corneal layer of the skin as well as hypotrichosis of the scalp and baldness (6, 10). Corneodesmosin is secreted by keratinocytes as a 52-56 kDa molecule which is then subjected to repeated sequential N- and C-terminal proteolysis (11). Species of 46, 43, 36, and 15 kDa are present in corneocytes (7, 11). Cleavage of the N-terminal Gly-loop diminishes Corneodesmosin’s ability to mediate adhesion, and this is a prerequisite for normal desquamation of the skin (8, 9). Reduced proteolysis of Corneodesmosin in psoriasis lesions is associated with the persistence of corneodesmosomes and scale retention (12). Premature truncation of Corneodesmosin is associated with hypotrichosis of the scalp (13).

• References:

1. Jonca, N. et al. (2011) Eur. J. Dermatol. 21 Suppl. 2:35.

2. Zhou, Y. and D.D. Chaplin (1993) Proc. Natl. Acad. Sci. 90:9470.

3. Haftek, M. et al. (1997) Br. J. Dermatol. 137:864.

4. Simon, M. et al. (1997) J. Biol. Chem. 272:31770.

5. Mils, V. et al. (1992) J. Histochem. Cytochem. 40:1329.

6. Matsumoto, M. et al. (2008) Proc. Natl. Acad. Sci. 105:6720.

7. Guerrin, M. et al. (1998) J. Biol. Chem. 273:22640.

8. Jonca, N. et al. (2002) J. Biol. Chem. 277:5024.

9. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:747.

10. Leclerc, E.A. et al. (2009) J. Cell Sci. 122:2699.

11. Simon, M. et al. (2001) J. Biol. Chem. 276:20292.

12. Simon, M. et al. (2008) Br. J. Dermatol. 159:77.

13. Levy-Nissenbaum, E. et al. (2003) Nat. Genet. 34:151.

• Entrez Gene IDs:

  1041 (Human); 386463 (Mouse)

• Alternate Names:

  CDSN; Corneodesmosin; D6S586E; differentiated keratinocyte S protein; HTSS; S protein; S