• Purity

  >95%, by SDS-PAGE with silver staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Mouse R?Spondin 3 (Catalog # ) is coated at 0.25 μg/ml, Recombinant Mouse ZNRF3 Fc Chimera binds with an apparent K    _d <0.2 nM.  

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse ZNRF3 (Lys53-Met216) Accession # Q5SSZ7	IEGRMDP	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Lys53

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  45 kDa

• SDS-PAGE

  51-60 kDa, reducing conditions

Data Images

Background: ZNRF3

ZNRF3 (zinc/RING finger protein 3) is a member of the ZNRF3 family of type 1 transmembrane ubiquitin ligases (1). Mature mouse ZNRF3 consists of a 163 amino acid (aa) extracellular domain (ECD) with a protease associated (PA) domain fold, a short transmembrane segment, and a 675 aa cytoplasmic tail that contains a RING-type zinc finger with E3 ubiquitin ligase activity. Within the ECD, mouse and human ZNRF3 share 98% sequence identity. ZNRF3 is co-expressed on the cell surface with the homologous protein RNF43. Both proteins serve to inhibit the Wnt signaling pathway through the ubiquitination of LRP6 and a majority of the Frizzled family of Wnt receptors (1, 4, 6). ZNRF3 ubiquitin ligase activity is regulated jointly by R-Spondin and its cell surface receptors, Lgr4, 5, and 6 (2). The R-Spondin-Lgr complex binds to and facilitates the removal of the ZNRF3 from the plasma membrane, resulting in an enhancement of Wnt signaling (3, 4). Conversely, ZNRF3 can antagonize R-Spondin enhanced Wnt signaling (3, 5). ZNRF3 is highly expressed in crypt stem cells of the intestine where it modulates Wnt-induced cell proliferation to control the turnover of intestinal epithelial cells (6). ZNRF3 expression is down-regulated in gastric carcinomas, and ZNRF3 mutations are linked to carcinomas of the gastric tract, pancreas, liver, and ovary (7-10).

• References:

1. de Lau, W. et al. (2014) Genes Dev. 28:305.

2. Xie, Y. et al. (2013) EMBO Rep. 14:1120.

3. Peng, W.C. et al. (2013) PLoS One 8:e83110.

4. Hao, H.X. et al. (2012) Nature 485:195.

5. Moad, H.E. and A.A. Pioszak (2013) Biochemistry 52:7295.

6. Koo, B.K. et al. (2012) Nature 488:665.

7. Ong, C.K. et al. (2012) Nat. Genet. 44:690.

8. Zhou, Y. et al. (2013) J. Mol. Histol. 44:555.

9. Ryland, G.L. et al. (2013) J. Pathol. 229:469.

10. Jiang, X. et al. (2013) Proc. Natl. Acad. Sci. USA 110:12649.

• Long Name:

  Zinc and Ring Finger 3

• Entrez Gene IDs:

  84133 (Human); 407821 (Mouse)

• Alternate Names:

  BK747E2.3; KIAA1133; novel C3HC4 type Zinc finger (ring finger); RNF203; zinc and ring finger 3; zinc/RING finger protein 3; ZNRF3