• Purity

  >95%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Mouse E-Cadherin Fc Chimera (Catalog # ) is coated at 2 μg/mL, Recombinant Mouse Integrin  alpha E beta 7 binds with an apparent K    _d <2.5 nM.  

• Source

  Chinese Hamster Ovary cell line, CHO-derived

  Mouse Integrin alpha E (Phe20-Arg1113, Ser453Gly) Accession # ABD49099	His-Pro	GGGSGGGS	Acidic Tail	6-His tag
  Mouse Integrin beta 7 (Glu20-Arg724) Accession # P26011	GGGSGGGS	Basic Tail
  N-terminus				C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Phe20 & Thr183 ( alpha E), Glu20 ( beta 7)

• Structure / Form

  Non-covalent heterodimer

• Predicted Molecular Mass

  130 kDa ( alpha E), 84 kDa ( beta 7)

• SDS-PAGE

  112-128 kDa and 142-176 kDa, reducing conditions

Background: Integrin alpha E beta 7

Integrin  alpha E beta 7 (also called M290 in mouse and HML-1 in human) is a type I transmembrane adhesion protein. It is composed of an alpha E subunit (epithelial-associated; also designated as CD103) which is expressed as disulfide-linked 150 kDa and 25 kDa heavy and light chains, and a non-covalently associated 130 kDa beta 7 glycoprotein subunit (1, 2). Each subunit has a transmembrane sequence and a short cytoplasmic tail. Integrin  alpha E beta 7 is the only known integrin family receptor containing the alpha E subunit, while the beta 7 subunit is also a component of Integrin  alpha 4 beta 7 (1-3). The alpha E extracellular domain (ECD) contains 7 beta -propeller domains surrounding an I domain followed by domains called tight, calf-1 and calf-2. An extra X domain, not found in any other alpha integrin, is also present and contains a proteolytic cleavage site (1, 2). The beta 7 ECD contains a vWFA domain, which interacts with the alpha E beta -propeller to form a binding domain. The MIDAS motif (metal ion dependent adhesion site) is critical for binding of alpha E beta 7 to its ligand, E-Cadherin (4). The 1093 amino acid (aa) mouse alpha E extracellular domain shares 79% and 99% aa sequence identity with human and rat alpha E respectively, while the 704 aa mouse beta 7 ECD shares 87% and 94% aa identity with human and rat beta 7, respectively. Integrin alpha E beta 7 is mainly restricted to mucosal tissues, where it engages E-Cadherin (4-6). It was first identified as a marker of intestinal intra-epithelial lymphocytes (1, 5, 6). It has since been recognized that a variety of leukocytes, such as cytotoxic CD8⁺ T cells, some dendritic cells, and effector/memory-like regulatory T cells, acquire Integrin  alpha E beta 7 in the days following their migration to epithelium in the intestines, lungs, and tonsils (6-13). In these tissues Integrin alpha E beta 7 facilitates immune surveillance, including the destruction of infected or transformed epithelial cells and the induction of T cell adaptive responses (7-13). Pathologically, Integrin alpha E beta 7 may be involved in allograft rejection of transplanted pancreatic islets and other tissues (14).

• References:

1. Shaw, S.K. et al. (1994) J. Biol. Chem. 269:6016.

2. Erle, D.J. et al. (1991) J. Biol. Chem. 266:11009.

3. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.

4. Higgins, J.M.G. et al. (2000) J. Biol. Chem. 275:25652.

5. Cepek, K.L. et al. (1994) Nature 372:190.

6. Wagner, N. et al. (1996) Nature 382:366.

7. Le Floc'h, A. et al. (2007) J. Exp. Med. 204:559.

8. Smyth, L.J.C. et al. (2007) Clin. Exp. Immunol. 149:162.

9. Woodberry, T. et al. (2005) J. Immunol. 175:4355.

10. Jaensson, E. et al. (2008) J. Exp. Med. 205:2139.

11. del Rio, M.-L. et al. (2008) J. Immunol. 181:6178.

12. Sung, S.J. et al. (2006) J. Immunol. 176:2161.

13. Siewert, C. et al. (2008) J. Immunol. 180:146.

14. Feng, Y. et al. (2002) J. Exp. Med. 196:877.

• Alternate Names:

  Integrin alpha E beta 7