• Purity

  >95%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to inhibit the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. The ED    ₅₀ for this effect is 0.1-0.6 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived Gly30-Gly498, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Gly30

• Predicted Molecular Mass

  51 kDa

• SDS-PAGE

  90-124 kDa, reducing conditions

Background: Endoglycan/PODXL2

Endoglycan, also called Podocalyxin-like 2 protein (PODXL2), is an extensively O-glycosylated type I transmembrane protein in the CD34 family of sialomucins (1, 2). Mature mouse Endoglycan consists of an extracellular domain (ECD) that contains an N-terminal acidic domain characterized by several polyglutamate tracts, a mucin domain, and a membrane-proximal globular domain. Within the ECD, mouse Endoglycan shares 96% and 75% amino acid identity with rat and human Endoglycan, respectively. Following the extracellular domain is a single transmembrane spanning helix and a charged cytoplasmic tail that contains a C-terminal PDZ?domain binding motif (1, 3). The cytoplasmic domain of Endoglycan has been shown to bind to Na  ⁺/H  ⁺ exchanger regulatory factor-1 (NHERF-1), which acts as a scaffolding protein that links membrane proteins to the actin cytoskeleton (4). Endoglycan exists as a disulfide-linked homodimer that is expressed in vascular endothelium, smooth muscle, hematopoietic precursor populations and leukocyte subpopulations (1, 5). Endoglycan functions through its interactions with selectins and is involved in leukocyte-endothelial cell adhesion although, depending on cellular context and post-translational modifications, Endoglycan may also demonstrate anti-adhesive properties (2, 3, 5, 6).

• References:

1. Sassetti, C. et al. (2000) J. Biol. Chem. 275:9001.

2. Fieger, C.B. et al. (2003) J. Biol. Chem. 278:27390.

3. Nielsen, J.S. and K.M. McNagny (2008) J. Cell Sci. 121:3683.

4. Tan, P.C. et al. (2006) Stem Cells 24:1150.

5. Kerr, S.C. et al. (2008) J. Immunol. 181:1480.

6. Sarangapani, K.K. et al. (2004) J. Biol. Chem. 279:2291.

• Entrez Gene IDs:

  50512 (Human); 319655 (Mouse)

• Alternate Names:

  Endoglycan; PODLX2; podocalyxin-like 2; podocalyxin-like protein 2; PODXL2