• Species Reactivity

  Mouse

• Specificity

  Detect mouse TGF-beta RI/ALK-5 in flow cytometry. Although this clone detects human and mouse TGF-beta RI/ALK-5 in Western blots, positive results have not been observed on human TGF-beta RI/ALK-5 in flow cytometry. In direct ELISAs and Western blots, this antibody shows no cross-reactivity with rrMIS RII, rhTGF? beta  RII, rhTGF? beta  RIIb, or rhTGF? beta  RIII.

• Source

  Monoclonal Rat IgG    _2A Clone # 141231

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant mouse TGF? beta  RI/ALK-5    
  Ala21-Glu121    
  Accession # BAA05023

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TGF-beta RI/ALK-5

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor, originally known as ALK-5 (Activin receptor-like kinase) is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

• References:

1. Miyazono, K. et al. (1994) Adv. In Immunol. 55:181.

2. Massagùe, J. (1998) Ann. Rev. Biochem. 67:753.

• Long Name:

  Transforming Growth Factor beta Receptor I

• Entrez Gene IDs:

  7046 (Human); 21812 (Mouse); 29591 (Rat)

• Alternate Names:

  AAT5; activin A receptor type II-like kinase, 53kD; activin A receptor type II-like kinase, 53kDa; Activin receptor-like kinase 5; ACVRLK4; ALK-5; ALK-5ALK5; EC 2.7.11; EC 2.7.11.30; LDS1A; LDS2A; Serine/threonine-protein kinase receptor R4; SKR4; tbetaR-I; TGFB1R1; TGF-beta receptor type I; TGF-beta receptor type-1; TGF-beta RI; TGF-beta type I receptor; TGFbetaRI; TGFBR1; TGF-bRI; TGFR-1; transforming growth factor beta receptor I; transforming growth factor, beta receptor 1; transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD); Transforming growth factor-beta receptor type I