• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <1.0 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 1 x 10    ⁵ cells/well are added to Recombinant Mouse P?Cadherin Fc Chimera coated plates (10 μg/mL with 100 μL/well), approximately 40?80% will adhere after 1.5 hours at 37 °C.    
     Optimal concentration depends on cell type as well as the application or research objectives.  

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse P-Cadherin (Glu100-Gly647) Accession # Q8BSL6	IEGRMD	Human IgG1 (Pro100-Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Glu100

• Predicted Molecular Mass

  87.8 kDa (monomer)

• SDS-PAGE

  100 kDa, reducing conditions

Background: P-Cadherin

Placental Cadherin (P-Cadherin, PCAD, or Cadherin-3) is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Constitutive P-Cadherin expression is found in the epidermis, mesothelium, corneal epithelium, and uterine decidua. Mouse P-Cadherin is an 822 amino acid (aa) protein with a 27 aa signal sequence and a 795 aa propeptide. The mature protein begins at aa 100 and has a 542 aa extracellular region, a 27 aa transmembrane region, and a 153 aa cytoplasmic region. Mutations in this gene have been associated with hypotrichosis with juvenile macular dystrophy (5). P-Cadherin over-expression is observed in various malignant tumors and is often correlated with increased tumor motility and invasiveness (6, 7).

• References:

1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.

2. Overduin, M. et al. (1995) Science 267:386.

3. Takeichi, M. (1991) Science 251:1451.

4. Nose, A. et al. (1987) EMBO J. 6:3655.

5. J. Invest. Derm. (2002) 199:1210.

6. Clin. Cancer Res. (2008) 14:6487.

7. Oncogene (2010) 29:393.

• Long Name:

  Placental Cadherin

• Entrez Gene IDs:

  1001 (Human); 12560 (Mouse)

• Alternate Names:

  CAD3; cadherin 3, P-cadherin (placental); cadherin 3, type 1, P-cadherin (placental); Cadherin-3; CDH3; CDHP; CDHPcalcium-dependent adhesion protein, placental; HJMD; PCAD; PCadherin; P-Cadherin; PCADP-cadherin; Placental cadherin