• Purity

  >95%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of EOMA mouse endothelial cells. The ED50 for this effect is 0.2-1 μg/mL

• Source

  Mouse myeloma cell line, NS0-derived Leu22-Ala786, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Leu22

• Predicted Molecular Mass

  83 kDa

• SDS-PAGE

  100-115 kDa, reducing conditions

Background: CD177

CD177 is a member of the uPAR/CD59/Ly6 superfamily (1). Mature mouse CD177 is a 796 amino acid (aa) protein that contains four uPAR/Ly6 domains, while human CD177 contains only two. Within common regions, mouse CD177 shares 55% and 77% aa sequence identity with human and rat CD177, respectively. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (2-4). It is nearly absent from neutrophils from paroxysmal nocturnal hemoglobinurea patients who are deficient in the ability to synthesize GPI linkages (4, 5). It is up-regulated on granulocytes from polycythemia vera and thalassemia patients (6, 7). CD177 binds to PECAM-1 on vascular endothelial cells, an interaction which mediates neutrophil adhesion to the vascular wall and neutrophil transmigration (8). It associates   in cis with the Integrin MAC-1 (CD11b/CD18) (9). CD177 also associates   in cis with Proteinase 3 (PR3) and is required for cell surface PR3 expression (9-11). PR3 is normally found in intracellular vesicles, but once at the cell surface it can serve as an autoimmune target for anti-neutrophil cytoplasmic antibodies (ANCA) (12). The ANCA targeting of CD177-PR3 complexes triggers neutrophil activation and vasculitis (9, 12).

• References:

1. Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.

2. Skubitz, K.M. et al. (1991) J. Leukoc. Biol. 49:163.

3. Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.

4. Klippel, S. et al. (2002) Blood 100:2441.

5. Boccuni, P. et al. (2000) Crit. Rev. Oncol. Hematol. 33:25.

6. Temerinac, S. et al. (2000) Blood 95:2569.

7. Zoi, K. et al. (2008) Brit. J. Haematol. 141:100.

8. Sachs, U.J.H. et al. (2007) J. Biol. Chem. 282:23603.

9. Jerke, U. et al. (2011) J. Biol. Chem. 286:7070.

10. von Vietinghoff, S. et al. (2007) Blood 109:4487.

11. Kuckleburg, C.J. et al. (2012) J. Immunol. 188:2419.

12. van Timmeren, M.M. and P. Heeringa (2012) Curr. Opin. Rheumatol. 24:8.

• Long Name:

  Polycythemia Rubra Vera Protein 1

• Entrez Gene IDs:

  57126 (Human); 68891 (Mouse)

• Alternate Names:

  CD177 antigenNB1 GP; CD177 molecule; CD177; HNA-2a; HNA2Acell surface receptor; Human neutrophil alloantigen 2a; NB1 GP; NB1PRV-1; polycythemia rubra vera 1; Polycythemia rubra vera protein 1; PRV1; PRV1NB1 glycoprotein