• Purity

  >95%, by SDS-PAGE with silver staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured in a cell proliferation assay using RT4?D6P2T rat schwannoma cells. The ED    ₅₀ for this effect is 0.1-0.5 μg/mL.

• Source

  E. coli-derived Glu27-Ala174, with an N-terminal Met

• Accession #

• N-terminal Sequence    
  Analysis

  Met-Glu27

• Predicted Molecular Mass

  16 kDa

• SDS-PAGE

  16 kDa, reducing conditions

Data Images

Background: Reg3G

Regenerating islet-derived protein 3-gamma (Reg3G), also known as pancreatitis-associated protein 3 (PAP3), is one of several secreted Reg proteins that contain a single C-type lectin domain and contribute to anti-microbial immunity in the gut (1, 2). Reg3G is secreted as a 148 amino acid (aa) proprotein, and 11 residues are cleaved from the N-terminus to generate the 15 kDa mature protein (3). This short propeptide serves as an inhibitor that limits the activity of the proprotein (3, 4). Mature mouse Reg3G shares 66% and 88% aa sequence identity with human and rat Reg3G, respectively. Reg3G is expressed by Paneth cells of the small intestine and is released into the gut lumen (5). It is up-regulated in gastrointestinal epithelium by bacterial inflammation or by defects in the mucous lining (5-8). Its expression and secretion is also induced in the lung in response to MRSA (drug resistant   S. aureus) and in damaged skeletal muscle and peripheral neurons (4, 9). Reg3G binds to bacterial peptidoglycans, exerts bacteriostatic and bactericidal activities, and protects the mucosa against bacterial invasion (4, 5, 10, 11). It also inhibits inflammation by limiting the number of Th1 cells in the lamina propria (11). R&D in-house data indicate that Reg3G can induce the proliferation of Schwann cells, a property it shares with Reg3B (12).

• References:

1. Parikh, A. et al. (2012) Biomol. Concepts 3:57.

2. Narushima, Y. et al. (1997) Gene 185:159.

3. Mukherjee, S. et al. (2009) J. Biol. Chem. 284:4881.

4. Choi, S-M. et al. (2013) J. Exp. Med. 210:551.

5. Cash, H.L. et al. (2006) Science 313:1126.

6. Lee, K.S. et al. (2012) PLoS ONE 7:e30786.

7. Keilbaugh, S.A. et al. (2005) Gut 54:623.

8. Burger-van Passen, N. et al. (2012) PLoS ONE 7:e38798.

9. Klasan, G.S. et al. (2014) Muscle Nerve 49:61.

10. Brandl, K. et al. (2007) J. Exp. Med. 204:1891.

11. Vaishnava, S. et al. (2011) Science 334:255.

12. Livesey, F.J. et al. (1997) Nature 390:614.

• Long Name:

  Regenerating Islet-derived III gamma

• Entrez Gene IDs:

  130120 (Human); 19695 (Mouse); 24620 (Rat)

• Alternate Names:

  LPPM429; MGC118999; MGC119001; Pancreatitis-associated protein 1B; Pancreatitis-associated protein IB; PAP IB; PAP1B; PAP-1B; PAP1BMGC118998; PAP3; PAPIB; PAPIII; Reg III-gamma; Reg3G; REG-3-gamma; regenerating gene III; regenerating islet-derived 3 gamma; regenerating islet-derived protein 3-gamma; Regenerating islet-derived protein III-gamma; REG-III; UNQ429