• Purity

  >95%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Mouse DC-SIGN is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse ICAM-5 Fc Chimera (Catalog # ) that produces 50% optimal binding response is 0.8-4 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived Val73-Lys238, with an N-terminal HA tag

• Accession #

• N-terminal Sequence    
  Analysis

  Tyr (HA tag)

• Predicted Molecular Mass

  20 kDa

• SDS-PAGE

  21-26 kDa, reducing conditions

Background: DC-SIGN/CD209

Mouse Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209, also known as CD209 Antigen-like Protein A, is a member of the C-type lectin family (1). Mouse DC-SIGN/CD209 is a 33 kDa, 238 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca  ²⁺-dependent carbohydrate-binding lectin domain (2). In addition to the full-length mouse DC-SIGN/CD209, a second, truncated splice variant has been reported. DC-SIGN/CD209 is not well conserved between mouse and human with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2-4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (2, 5, 6).

• References:

1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.

2. Caminschi, I. et al. (2001) Mol. Immunol. 38:365.

3. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.

4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.

5. Geijtenbeek, T.B. et al. (2000) Cell 100:575.

6. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.

• Long Name:

  Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1

• Entrez Gene IDs:

  30835 (Human); 170786 (Mouse)

• Alternate Names:

  CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DCSIGN; DC-SIGN; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1