• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3?E1 mouse preosteoblast cells. The ED    ₅₀ for this effect is 10-60 ng/ml in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (Catalog # ).

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse Frizzled-5 (Ala27-Pro167) Accession # Q9EQD0	IEGRMD	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Ala27

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  43 kDa

• SDS-PAGE

  54-64 kDa, reducing conditions

Data Images

Background: Frizzled-5

Frizzled-5 is an approximately 62 kDa 7-TM glycoprotein that is one of at least ten Frizzled family Wnt receptors (1, 2). Wnt engagement of Frizzled, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co?receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance (1). Frizzleds can also signal through non-canonical pathways independently of LRPs (1). Mature mouse Frizzled-5 consists of a 212 amino acid (aa) extracellular domain (ECD), a 283 aa 7-TM region, and a 64 aa cytoplasmic domain with a PDZ binding motif (2, 3). The ECD includes a cysteine-rich region (CRD) that binds Wnts and is highly conserved among Frizzled proteins (1, 2, 4). Within the N-terminal ECD, human Frizzled-5 shares 95% aa sequence identity with mouse and rat Frizzled-5. Frizzled-5 is expressed in embryonic tissues (telencephalon, pituitary, thalamus, hypothalamus, eye, liver, spleen, lung, and kidney), in the adult retina, colon, and pancreatic islets, some cancer cell lines, human embryonic stem cells, and in some monocyte and lymphocyte populations (2-8). Frizzled-5 functions as a receptor for Wnt-5a, Wnt-9b, Wnt-10b, Wnt-2b, and Wnt-7a (3, 5, 9, 10). It plays a role in the maintenance of yolk sac and placental vasculature (ishikawa) and in the regression of vitreous vasculature during eye development (5, 6, 11). It mediates the synaptogenic effect of Wnt-7a, contributes to the development of neuronal polarity, and is required for neuronal survival in the thalamus (3, 12, 13). In macrophages and monocytes, Frizzled-5 signaling induces the production of inflammatory cytokines (7, 8).

• References:

1. Schulte, G. (2015) Eur. J. Pharmacol. 763:191.

2. Saitoh T. et al. (2001) Int. J. Oncol. 19:105.

3. Liu, C. et al. (2008) J. Neurosci. 28:5641.

4. Katoh, Y. and M. Katoh (2007) Int. J. Oncol. 30:751.

5. Ishikawa, T. et al. (2001) Development 128:25.

6. Burns, C.J. et al. (2008) Dev. Dyn. 237:1614.

7. Periera, C. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:504.

8. Blumenthal, A. et al. (2006) Blood 108:965.

9. He, X. et al. (1997) Science 275:1652.

10. Caricasole, A. et al. (2003) J. Biol. Chem 278:37024.

11. Zhang, J. et al. (2008) Invest. Ophthalmol. Vis. Sci. 49:5561.

12. Sahores, M. et al. (2010) Development 137:2215.

13. Slater, P.G. et al. (2013) PLoS One 8:e78892.

• Entrez Gene IDs:

  7855 (Human); 14367 (Mouse)

• Alternate Names:

  C2orf31; chromosome 2 open reading frame 31; DKFZP434E2135; frizzled (Drosophila) homolog 5; frizzled homolog 5 (Drosophila); Frizzled5; Frizzled-5; Frz-5; FZ-5; FZD5; fzE5; HFZ5; MGC129692; seven-transmembrane receptor frizzled-5; Wnt receptor