• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to chemoattract BaF3 mouse pro?B cells transfected with human CXCR2. The ED    ₅₀ for this effect is 3-15 ng/mL.

• Source

  E. coli-derived Ala29-Asn101

• Accession #

• N-terminal Sequence    
  Analysis

  Ala29

• Predicted Molecular Mass

  8.0 kDa

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: CXCL2/GRO beta/MIP-2/CINC-3

Macrophage inflammatory protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. The protein is produced by a variety of cell types, including intestinal epithelium, macrophages, astrocytes and fibroblasts. Homologs of the mouse MIP-2 have been identified in human, rat and cotton rat. MIP-2 is an ELR-containing member of the alpha (CXC) subfamily of chemokines. The cotton rat MIP-2 gene encodes a 101 amino acid (aa) residue precursor protein with a 28 aa putative signal peptide that is cleaved to generate the 73 aa mature protein. Mature cotton rat MIP-2 has two intrachain disulfide bonds and no potential glycosylation sites. It shares approximately 79% and 85% aa sequence identity with rat CINC-3 and mouse MIP-2, respectively. MIP-2 is a potent neutrophil attractant and activator. MIP-2 binds and activates the chemokine receptor CXCR2. The recombinant cotton rat MIP-2 has been shown to bind and activate the human CXCR2 (1 - 5).

• References:

1. Yoshie, O. et al. (2001) Adv. Immunol. 78:57.

2. Nakagawa, H. et al. (1994) Biochem. J. 301:545.

3. Telkamp-Olson, P. et al. (1990) J. Exp. Med. 172:911.

4. Lee, J. et al. (1995) J. Immunol. 155:2158.

5. Feng, L. et al. (1995) J. Clin. Invest. 95:1009.

• Entrez Gene IDs:

  2920 (Human); 20310 (Mouse); 114105 (Rat)

• Alternate Names:

  chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROB; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2