• Purity

  >97%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to chemoattract BaF3 mouse pro?B cells transfected with mouse CXCR3. The ED    ₅₀ for this effect is 2-10 μg/mL.

• Source

  E. coli-derived Ile22-Val94, with an N-terminal Met

• Accession #

• N-terminal Sequence    
  Analysis

  Met

• Predicted Molecular Mass

  8.6 kDa

Background: CXCL10/IP-10/CRG-2

P-10, also named mob-1 in rat and crg-2 in mouse, is a member of the non-ELR-motif containing CXC chemokine subfamily and has been designated CXCL10. It is induced in various cell types (including keratinocytes, endothelial cells, fibroblasts, smooth muscle cells, monocytes/macrophages, astrocytes, neutrophils, hepatocytes, T cells, epithelial cells and mesothelial cells) in response to stimulation by type I and/or type II interferon as well as by LPS. IP-10 has been shown to chemoattract activated CD4+ and CD8+ T cells, activated NK cells and smooth muscle cells. It also induces the adhesion of activated T cells to VCAM and ICAM. In addition, IP-10 has been reported to antagonize the angiogenic activity of FGF. IP-10 binds to and activates CXCR3, the chemokine receptor that is shared by MIG/CXCL9 and I-TAC/CXCL11, two additional non-ELR CXC chemokines that are induced by interferon. Cotton rat IP-10 cDNA encodes a 94 amino acid (aa) residue precursor protein with a putative 21 aa signal peptide and a 73 aa mature protein with one potential N-linked glycosylation site. Mature cotton rat IP-10 shares 66%, 64%, and 66% aa sequence identity with rat, mouse and human IP10, respectively (1 - 7).

• References:

1. Vanguri, P. and J.M. Farber (1990) J. Biol. Chem. 265:15049.

2. Liang, P. et al. (1994) Proc. Natl. Acad. Sci. USA. 91:12515.

3. Proost, P. et al. (1993) J. Immunol. 150:1000.

4. Blanco, J.C. et al. (2001) GenBank Accession # AAL16959.

5. Neville, L.F. (1997) Prog. Growth Factor Res. 8:207.

6. Belperio, J.A. et al. (2000) J. Leukoc. Biol. 68:1.

7. Soejima, K. and B.J. Rollins (2001) J. Immunol. 167:6576.

• Entrez Gene IDs:

  3627 (Human); 15945 (Mouse)

• Alternate Names:

  C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10