• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured in a cell proliferation assay using 4MBr?5 rhesus monkey epithelial cells. Rubin, J.S.     et al. (1989) Proc. Natl. Acad. Sci. USA     86:802. The ED    ₅₀ for this effect is 25-50 ng/mL.

• Source

  E. coli-derived Cys32-Thr194 & Ser55-Thr194

• Accession #

• N-terminal Sequence    
  Analysis

  Cys32 & Ser55    
   

• Predicted Molecular Mass

  18.8 kDa

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: KGF/FGF-7

The fibroblast growth factor (FGF) family consists of twenty three known structurally-related proteins that play key roles in development, morphogenesis, angiogenesis, wound healing and tumorigenesis (1). All FGFs share a common structural fold, the beta -trefoil scaffold, in a conserved central region of approximately 120 amino acid (aa), which contains the active site and a heparin binding site (2). FGF-7, also known as keratinocyte growth factor (KGF), was originally identified from the conditioned medium of a human embryonic lung fibroblast cell line (3). It is secreted by cells of mesenchymal origin and functions predominantly on epithelial cells (1). The mature caFGF-7 is generated from a 194 aa precursor protein by cleavage of the first 31 aa at the N-terminus. It shares 97.4% and 95.9% sequence identity with human and mouse KGF, respectively (4). FGFs signal through high affinity tyrosine kinase receptors (FGF R) (5). Cellular responses to FGFs are modulated by heparan sulfate proteoglycans that are also known as low affinity FGF receptors and by heparin (6). Various isoforms of the four FGF R exist (FGF R?1 through -4). They are produced from alternatively spliced transcripts in both the extracellular and intracellular domains. FGF-7 signals through the FGF R2IIIb (also known as KGF R), which is a spliced variant of FGF R2 (7). KGF R binds FGF-1 and FGF-7 with high affinity and FGF-2 with a 20-fold lower affinity (8).

• References:

1. Zetter, B.R. (1998) Annu. Rev. Med. 49:407.

2. Murzin, A.G. et al. (1992) J. Mol. Biol. 223:531.

3. Finch, P.W. et al. (1989) Science 245:752.

4. Canatan, H. et al. (1996) DNA Cell Biol. 15:247.

5. Vlodavsky, I. et al. (1996) Cancer Metastasis Rev. 15:177.

6. Givol, D. and A. Yayon (1992) FASEB J. 6:3362.

7. Miki, T. et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89:246.

8. Bottaro, D.P. et al. (1990) J. Biol. Chem. 265:12767.

• Long Name:

  Keratinocyte Growth Factor

• Entrez Gene IDs:

  2252 (Human); 403915 (Canine)

• Alternate Names:

  FGF7; FGF-7; fibroblast growth factor 7HBGF-7; Heparin-binding growth factor 7; keratinocyte growth factor; KGF; KGFfibroblast growth factor 7 (keratinocyte growth factor)