• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to synergize with Recombinant Mouse Wnt?3a (Catalog # ) to stabilize beta -catenin protein levels in HEK293T human embryonic kidney cells as assessed with Western blot. This effect is detected at doses as low as 1 ng/mL in the presence of 20 ng/mL recombinant mouse Wnt-3a. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED    ₅₀ is 0.7-2.8 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.

• Source

  Mouse myeloma cell line, NS0-derived Gln22-Gly205, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained: Gln22 predicted

• Predicted Molecular Mass

  22 kDa

• SDS-PAGE

  25-29 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Data Images

Background: R-Spondin 2

Roof plate-specific Spondin 2 isoform 1 (R?Spondin 2, RSPO2), also known as cysteine?rich and single thrombospondin domain containing protein 2 (Cristin 2), is a 33 kDa secreted protein that belongs to the R-Spondin family (1?3). The four R?Spondins regulate Wnt/ beta -catenin signaling and overlap in expression and function (1?3). Like other R?Spondins, RSPO2 contains two adjacent cysteine?rich furin-like domains (aa 90?134) followed by a thrombospondin (TSP-1) motif (aa 144?204) and a C-terminal region rich in basic residues (aa 207?243). The basic region binds heparin and mediates cell surface retention and extracellular matrix attachment while the furin?like domains are required for Wnt/ beta -catenin signaling (1, 3, 4). RSPO2 contains one potential N?glycosylation site. Mature human RSPO2 shares 97?98% aa identity with mouse, rat, equine, canine and bovine RSPO2 and ~40% aa identity with RSPO1, RSPO3 and RSPO4. Of the three reported splice isoforms of human R?Spondin 2, isoform 2 lacks residues 1 ? 67 of isoform 1, while isoform 3 has a glycine substitution for residues 32?95 of isoform 1 (5). Human RSPO2 is expressed in organs of endodermal origin in adults, including intestine and lung, and is down?regulated in tumors of these tissues (1). In the embryonic mouse, RSPO2 expression is concentrated in the apical epidermal ridge, hippocampus, and developing muscle, teeth and bones (1, 6). Deletion of RSPO2 results in down?regulation of Wnt activity in these areas, malformations of the facial skeleton and limbs, and respiratory failure at birth (7?9). RSPO2 is an extracellular potentiator of Wnt/ beta -catenin signaling (3, 4). It functions at least in part by binding LRP?6, stimulating its long-term phosphorylation and down?regulating its internalization (3, 4). RSPO proteins, especially RSPO2 and RSPO3, also antagonize DKK1 activity by interfering with DKK1?mediated LRP?6 and Kremen association (10).

• References:

1. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.

2. Kim, K.-A. et al. (2006) Cell Cycle 5:23.

3. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.

4. Li, S-J. et al. (2009) Cell Signal. 21:916.

5. Swiss-Prot Accession # Q6UXX9.

6. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.

7. Yamada, W. et al. (2009) Biochem. Biophys. Res. Commun. 381:453.

8. Jin, Y.-R. et al. (2011) Dev. Biol. 352:1.

9. Nam, J.-S. et al. (2007) Dev. Biol. 311:124.

10. Kim, K.-A. et al. (2007) Mol. Biol. Cell 19:2588.

• Long Name:

  Roof Plate-specific Spondin 2

• Entrez Gene IDs:

  340419 (Human); 239405 (Mouse)

• Alternate Names:

  Cristin 2; CRISTIN2; hRspo2; MGC35555; MGC43342; Roof plate-specific spondin-2; RSPO2; R-spondin 2 homolog (Xenopus laevis); RSpondin 2; R-Spondin 2; R-spondin-2