• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit proliferation of the A549 human lung carcinoma cells. The ED    ₅₀ for this effect is 100-400 ng/mL.

• Source

  Mouse myeloma cell line, NS0-derived

  Human Semaphorin 5A (Glu23-Thr765) Accession # AAC09473	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Glu23

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  110 kDa (monomer)

• SDS-PAGE

  133 kDa, reducing conditions

Background: Semaphorin 5A

Semaphorin 5A (Sema5A, previously called SemaF) is a 140 kDa protein of the semaphorin family of axon guidance molecules (1-4). Class 5 semaphorins are type I transmembrane glycoproteins with an N-terminal Sema domain and multiple juxtamembrane type 1 thrombospondin (TSP) repeats within their extracellular domains (1?3). Sema5A is expressed developmentally in oligodendrocytes, neuroepithelial cells surrounding retinal axons, the base of limb buds, the cardiac atrial septum and endocardial cushions, and the mesoderm surrounding cranial vessels (4-6). The human Sema5A cDNA encodes a 22 amino acid (aa) signal sequence, a 946 aa extracellular domain (ECD), a 22 aa transmembrane sequence and an 85 aa cytoplasmic portion. Within aa 23 - 765, which includes the sema domain and four of the seven TSP repeats, human Sema5A shares 93% aa identity with corresponding mouse, rat, and canine sequences. Semaphorins typically transduce signals through transmembrane plexins (1, 2). The sema domain of Sema5A binds plexin B3, triggering signaling via HGF R/c-Met (7). Both Sema5A and plexin B3 are expressed postnatally during differentiation and migration of central nervous system oligodendrocytes. However, plexin B3 is not significantly expressed prenatally and therefore unlikely to be the Sema5A receptor during development (7, 8). The Sema5A TSP repeats interact with either heparin sulfate or chondroitin sulfate proteoglycans (HSPG, CSPG) (9). HSPG interaction promotes attraction, while CSPG interaction promotes repulsion and is essential for axon fasciculation, independent of plexin B3 (9, 10). Sema5A mutations have been implicated in the genetic syndrome, cri-du-chat, while some polymorphisms may increase risk for neurodegenerative diseases such as Parkinson’s (3, 11). Sema5A expression may be upregulated in metastatic cancer cells and downregulated in autism (12, 13).

• References:

1. Flannery, E. and M. Duman-Scheel (2009) Curr. Drug Targets 10:611.

2. Zhou, Y. et al. (2008) Trends Biol. Sci. 33:161.

3. Simmons, A.D. et al. (1998) Biochem. Biophys. Res. Commun. 242:685.

4. Oster, S.F. et al. (2003) Development 130:775.

5. Goldberg, J.L. et al. (2004) J. Neurosci. 24:4989.

6. Fiore, R. et al. (2005) Mol. Cell. Biol. 25:2310.

7. Artigiani, S. et al. (2004) EMBO Rep. 5:710.

8. Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.

9. Kantor, D.B. et al. (2004) Neuron 44:961.

10. Hilario, J.D. et al. (2008) Dev. Biol. 326:190.

11. Lin, L. et al. (2009) Trends Neurosci. 32:142.

12. Pan, G-Q. et al. (2009) World J. Gastroenterol. 15:2800.

13. Melin, M. et al. (2006) Neuropsychobiology 54:64.

• Entrez Gene IDs:

  9037 (Human); 20356 (Mouse); 310207 (Rat)

• Alternate Names:

  FLJ12815; sema domain, seven thrombospondin repeats (type 1 and type 1-like); Sema F; SEMA5A; SEMAF; Semaphorin 5A; semaphorin F; semaphorin-5A; Semaphorin-F; semF; transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A