• Purity

  >95%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Human APP695 is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Human NgR3/NgRH2 that produces 50% optimal binding response is 0.05-0.25 μg/mL.

• Source

  Human embryonic kidney cell, HEK293-derived Cys25-Ala419, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Cys25

• Predicted Molecular Mass

  45 kDa

• SDS-PAGE

  56-65 kDa, reducing conditions

Background: NgR3/NgRH2

NgR3/NgRH2 (also known as reticulon-4 receptor-Like 1), along with the related NgR1 and NgR2, is a member of the Nogo66 receptor (NgR) family. NgR proteins are expressed primarily by neurons in the central and peripheral mammalian nervous system. NgR proteins play an essential role in mediating axon growth inhibition induced by the structurally distinct proteins Nogo, myelin associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (OMgp) (1, 2).These proteins belong to the family of myelin-associated inhibitors (MAIs). NgR proteins are composed of 8 leucine-rich repeats (LRRs) flanked by N-terminal and C-terminal leucine-rich capping domains and are attached at the cell surface by a C-terminal glycosylphosphatidylinositol (GPI) anchor (3). Because NgR proteins have no transmembrane domain, they must be associated with membrane proteins in order to transduce cell signaling (2). Both NGF R/TNFRSF16 (p75) and TROY/TNFRSF19 have been identified as NgR1-associated components (4, 5). Human NgR3 shares 88% and 86% amino acid sequence identity with mouse and rat NgR3 respectively. NgR1 and NgR3 serve as receptors for chondroitin sulfate proteoglycans (CSPGs) (6). NgR3 may function as a NgR1 co-receptor for Nogo66 (7). CSPGs and MAIs are known to stabilize neuronal structure and influence the growth and guidance of developing neurons (8). Therefore, NgR proteins are part of a multicomponent receptor system implicated in pathways that limit neuronal growth and increase the structural stability of synapses.

• References:

1. GrandPre, T. et al. (2002) Nature 417:547.

2. Wang, K.C. et al. (2002) Nature 420:74.

3. Fournier, A.E. et al. (2001) Nature 409:341.

4. Shao, Z. et al. (2005) Neuron 45:353.

5. Park, J. B. et al. (2005) Neuron 45:345.

6. Dickendesher, T.L. et al. (2012) Nat. Neurosci. 15:703.

7. Zang, G. et al. (2011) J. Genet. Genomics 38:515.

8. Kantor, D.B. et al. (2004) Neuron 44:961.

• Long Name:

  Nogo-66 Receptor-related Protein 3/Nogo-66 Receptor Homolog 2/Reticulon-4 Receptor-like 1

• Entrez Gene IDs:

  146760 (Human); 237847 (Mouse)

• Alternate Names:

  DKFZp547J144; NgR3; NgRH2; NGRL2; nogo receptor-like 2; Nogo-66 receptor homolog 2; Nogo-66 receptor-related protein 3; reticulon 4 receptor-like 1; reticulon-4 receptor-like 1; RTN4RL1