• Purity

  >95%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit IL-22-induced IL-10 secretion by COLO 205 human colorectal adenocarcinoma cells. The ED    ₅₀ for this effect is typically 1-5 ng/mL in the presence of 1 ng/mL of Recombinant Human IL-22 (Catalog # ).

• Source

  Chinese Hamster Ovary cell line, CHO-derived Thr22-Pro231, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Thr22

• Predicted Molecular Mass

  26 kDa

• SDS-PAGE

  46-53 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: IL-22BP

Interleukin 22 binding protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35-45 kDa secreted glycoprotein in the type II cytokine receptor family (CRF). IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is also a component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29 (1, 2). IL-22BP blocks the interaction of IL-22 with IL-22 R, preventing IL-22 induced production of reactive oxygen species, IL-6, IL-10, and TNF-a (3-8).   In vivo, it regulates the proinflammatory effects of IL-22 (  e.g. neutrophil infiltration) but not of IL-10 (7). Mouse IL-22BP can neutralize the bioactivity of both mouse and human IL-22 (6). IL-22BP is produced by dendritic cells (DC), epithelial cells, activated B cells, and activated monocytes (3, 6, 9, 10). It is constitutively expressed by DC but is down-regulated during local inflammation and in response to tissue damage (11-13). IL-22BP is critical for limiting IL-22 induced epithelial cell proliferation during wound healing, and its deficiency can enable uncontrolled proliferation and enhance tumor development (12). Mature human IL-22BP contains two Fibronectin type III domains (3, 4). Alternative splicing generates additional isoforms that contain a 32 amino acid (aa) insertion in the first Fn-III domain and may also be truncated within the second Fn-III domain (3, 4, 14). Human IL-22BP without the 32 aa insertion shares 68% and 73% amino acid (aa) sequence identity with mouse and rat IL-22BP, respectively.

• References:

1. Lim, C. and R. Savan (2014) Cytokine Growth Factor Rev. 25:257.

2. Mizoguchi, A. (2012) Inflamm. Bowel Dis. 18:1777.

3. Xu, W. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9511.

4. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.

5. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.

6. Wei, C.-C. et al. (2003) Genes Immun. 4:204.

7. Weber, G.F. et al. (2007) Infect. Immun. 75:1690.

8. Whittington, H.A. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:220.

9. Lecart, S. et al. (2002) Int. Immunol. 14:1351.

10. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.

11. Wolk, K. et al. (2007) J. Immunol. 178:5973.

12. Huber, S. et al. (2012) Nature 491:259.

13. Martin, J.C.J. et al. (2014) Mucosal Immunol. 7:101.

14. Dumoutier, L. et al. (2001) J. Immunol. 166:7090.

• Long Name:

  Interleukin 22 Binding Protein

• Entrez Gene IDs:

  116379 (Human); 237310 (Mouse)

• Alternate Names:

  class II cytokine receptor; CRF2-10; CRF2-S1IL22BP; CRF2-X; Cytokine receptor class-II member 10; Cytokine receptor family 2 member 10; Cytokine receptor family type 2, soluble 1; IL-22 RA2; IL-22 receptor subunit alpha-2; IL22BP; IL-22BP; IL-22BPCRF2X; IL22RA2; IL-22RA2; IL-22R-alpha-2; interleukin 22 receptor, alpha 2; interleukin 22-binding protein; interleukin-22 receptor subunit alpha-2; Interleukin-22-binding protein; MGC150509; MGC150510; zcytoR16