• Purity

  >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to bind Recombinant Human NKG2D/CD314 Fc Chimera (Catalog # ) in a functional ELISA.

• Source

  Mouse myeloma cell line, NS0-derived

  Human ULBP-2 (Gly26-Ser217) Accession # Q9BZM5	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Gly26

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  48 kDa (monomer)

• SDS-PAGE

  60-65 kDa, reducing conditions

Background: ULBP-2

ULBP-2 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. The name ULBP-2 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-2 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30 – 60%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. ULBP-2 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance.

• References:

1. Cosman, D. et al. (2001) Immunity 14:123.

2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.

3. Sutherland, C. et al. (2002) J. Immunol. 168:671.

4. Steinle, A. et al. (2001) Immunogenetics 53:279.

5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.

6. Pende, D. et al. (2002) Cancer Res. 62:6178.

7. Radosavljevic, M. et al. (2002) Genomics 79:114.

8. NKG2D and its Ligands (2002) www.rndsystems.com.

• Long Name:

  UL16 Binding Protein-2

• Entrez Gene IDs:

  80328 (Human)

• Alternate Names:

  ALCAN-alpha; N2DL2; N2DL-2; NKG2D ligand 2; NKG2DL2; RAET1H; RAET1HNKG2DL2; retinoic acid early transcript 1 H; Retinoic acid early transcript 1H; UL16 binding protein 2; UL16-binding protein 2; ULBP2; ULBP-2