• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to bind its ligand, rhMICA/Fc Chimera in a functional ELISA.

• Source

  Mouse myeloma cell line, NS0-derived

  MD	Human IgG1 (Pro100-Lys330)	IEGR	Human NKG2D (Phe78-Val216) Accession # P26718
  N-terminus			C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Met

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  43 kDa (monomer)

• SDS-PAGE

  50-55 kDa, reducing conditions

Background: NKG2D/CD314

NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8⁺ alpha beta T cells, gamma δ T cells, NK cells and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.

• References:

1. Li, P. et al. (2001) Nature Immunol. 2:443.

2. Steinle, A. et al. (2001) Immunogenetics 53:279.

3. Cosman, D. et al. (2001) Immunity 14:123.

4. Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.

5. Wu, J. et al. (1999) Science 285:730.

6. Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.

7. Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.

8. Groh, V. et al. (2001) Nature Immunol. 2:255.

9. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.

10. Diefenbach, A. et al. (2001) Nature 413:165.

11. NKG2D and its Ligands (2002) www.rndsystems.com

• Long Name:

  Natural Killer G2D

• Entrez Gene IDs:

  22914 (Human); 27007 (Mouse)

• Alternate Names:

  CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2D; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence