• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells. The ED    ₅₀ for this effect is typically 150-900 ng/mL in the presence of 300 ng/mL of Recombinant Mouse Wnt?3a (Catalog # ).

• Source

  Human embryonic kidney cell, HEK293-derived Ser32-Lys314

• Accession #

• N-terminal Sequence    
  Analysis

  Ser32

• Predicted Molecular Mass

  33 kDa

• SDS-PAGE

  37-43 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: sFRP-1

Secreted Frizzled Related Protein-1 (sFRP-1), also known as SARP-2, FRP, and FrzA, belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30-35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Mature mouse sFRP-1 shares 97% amino acid sequence identity with human and rat sFRP-1. It binds to Wnt-1, -2, -3a, and -4 and prevents their activation of canonical beta-catenin signaling (4-7). sFRP-1 is up-regulated in the ischemic heart where it limits inflammation and the severity of tissue damage following myocardial infarction (8-10). During angiogenesis it induces endothelial cell migration, tube formation, and vessel formation (11). sFRP-1 is expressed by bone marrow stromal cells and osteoclasts (12). It binds to TRANCE/RANK Ligand, inhibits osteoclast and osteoblast formation and survival, and inhibits the formation of trabecular bone (13, 14). sFRP-1 additionally restricts kidney tubule formation (7), the inflammatory activation of leukocytes (10), early B lymphoid progression (12), and vitreous fluid outflow in the eye (15).

• References:

1. Cruciat, C.M. and C. Niehrs (2013) Cold Spring Harb. Perspect. Biol. 5:e015081.

2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.

3. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2859.

4. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.

5. Dennis, S. et al. (1999) J. Cell Sci. 112:3815.

6. Wawrzak, D. et al. (2007) Biochem. Biophys. Res. Commun. 357:1119.

7. Yoshino, K. et al. (2001) Mech. Dev. 102:45.

8. Barandon, L. et al. (2002) Circulation 108:2282.

9. Sklepkiewicz, P. et al. (2015) Circ. Heart Fail. 8:362.

10. Barandon, L. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:e80.

11. Dufourcq, P. et al. (2002) Circ. Res. 106:3097.

12. Yokota, T. et al. (2008) J. Immunol. 181:6061.

13. Hausler, K.D. et al. (2004) J. Bone Miner. Res. 19:1873.

14. Bodine, P.V.N. et al. (2004) Mol. Endocrinol. 18:1222.

15. Wang, W.-H. et al. (2008) J. Clin. Invest. 118:1056.

• Long Name:

  Secreted Frizzled Related Protein 1

• Entrez Gene IDs:

  6422 (Human); 20377 (Mouse); 84402 (Rat)

• Alternate Names:

  FRP1; FRP-1FrzA; FRPSARP-2; FrzA; SARP-2; SARP2sFRP-1; Secreted apoptosis-related protein 2; secreted frizzled-related protein 1; sFRP1; sFRP-1