• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey IgG    ₁ Fc is immobilized at 0.15 μg/mL (100 μL/well), the concentration of Recombinant Human Fc gamma  RI/CD64 (Catalog # ) that produces 50% of the optimal binding response is 0.2-1.2 ng/mL.

• Source

  Human embryonic kidney cell, HEK293-derived Pro110-Lys333

• Accession #

• N-terminal Sequence    
  Analysis

  Pro110, and Ala-Leu-Ala-Pro110

• Predicted Molecular Mass

  25 kDa

• SDS-PAGE

  30-40 kDa, reducing conditions

Data Images

Background: IgG1

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in human adult blood. It is composed of two heavy chains and two light chains. The variability of the IgG pool is generated by somatic recombination in B cells that have been exposed to foreign material. IgA, IgG, IgM, IgD, and IgD classes of human immunoglobulin (as well as the isotypes IgG1, IgG2a, IgG2b, and IgG3) are generated by class switch recombination within the heavy chain locus (1). Recombination within the light chain locus selects either kappa or lambda chains. Antibodies are secreted by plasma cells of the B lymphocyte lineage and constitute the humoral component of adaptive immunity (2, 3). Their variable regions bind to foreign antigens, while their Fc regions are recognized by Fc receptors expressed on macrophages, monocytes, neutrophils, NK cells, and CD8  ⁺ cytotoxic T cells (4). Phagocyte uptake of antibody-antigen complexes and antibody-opsonized pathogens is critical for clearance of the foreign material and immune homeostasis.

• References:

1. Stavnezer, J. et al. (2008) Annu. Rev. Immunol. 26:261.

2. Nutt, S.L. et al. (2015) Nat. Rev. Immunol. 15:160.

3. Swanson, C.L. et al. (2013) Immunol. Res. 55:277.

4. Nagelkerke, S.Q. and T.W. Kuijpers (2015) Front. Immunol. 5:674.

• Long Name:

  Immunoglobulin G1

• Entrez Gene IDs:

  101867415 (Cynomolgus Monkey)

• Alternate Names:

  IgG1