• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED    ₅₀ for this effect is 0.9-4.5 μg/mL.    
   

• Source

  Human embryonic kidney cell, HEK293-derived Gln35-His424, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained. Gln35 inferred from enzymatic pyroglutamate treatment revealing Val36

• Predicted Molecular Mass

  45 kDa

• SDS-PAGE

  62-73 kDa, reducing conditions

Data Images

Background: PSG-6

Pregnancy-specific glycoprotein 6 (PSG-6), also known as PSG10 and PSGGB is a secreted glycoprotein of the PSG family within the CEA (carcinoembryonic antigen) superfamily (1). Mature human PSG-6 is a 401 amino acid (aa) protein that consists of four tandem immunoglobulin-like domains followed by a 30 aa  
C-terminal tail (2, 3). The N-terminal Ig-like domain contains an Arg-Gly-Asp (RGD) sequence which is a consensus integrin-binding motif found in many adhesion proteins (4, 5). Alternative splicing generates an additional isoform with a substitution in the C-terminal tail. PSG-6 is predominantly expressed in placenta and more weakly in granulocytes (3). It induces monocyte production of the cytokines IL-6, IL-10, and TGF-beta 1 (6).

• References:

1. Kuespert, K. et al. (2006) Curr. Opin. Cell Biol. 18:565.

2. Zimmermann, W. et al. (1989) Biochem. Biophys. Res. Commun. 163:1197.

3. Barnett, T.R. et al. (1990) Biochemistry 29:10213.

4. Leslie, K.K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5822.

5. Ruoslahti, E. (1996) Annu. Rev. Cell Dev. Biol. 12:697.

6. Snyder, S.K. et al. (2001) Am. J. Reprod. Immunol. 45:205.

• Long Name:

  Pregnancy-specific beta-1-glycoprotein 6

• Entrez Gene IDs:

  5675 (Human)

• Alternate Names:

  CGM3; pregnancy specific beta-1-glycoprotein 6; Pregnancy-specific beta-1-glycoprotein 10; Pregnancy-specific beta-1-glycoprotein 12; pregnancy-specific beta-1-glycoprotein 6; Pregnancy-specific glycoprotein 10; Pregnancy-specific glycoprotein 12; Pregnancy-specific glycoprotein 6; PS-beta-G-10; PS-beta-G-12; PS-beta-G-6; PSBG-10; PSBG-12; PSBG-6; PSG10; PSG12; PSG6; PSG-6; PSGGB