• Purity

  >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Neu5Ac(a2-6)GalNAc-PAA-biotin is immobilized onto a Streptavidin-coated plate, Recombinant Human Siglec-15 Fc Chimera binds with an ED    ₅₀ of 150-900 ng/mL.

• Source

  Chinese Hamster Ovary cell line, CHO-derived

  Human Siglec-15 (Phe20-Thr263) Accession # Q6ZMC9	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Phe20

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  53 kDa

• SDS-PAGE

  53-64 kDa, reducing conditions

Data Images

Background: Siglec-15

Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain (2). Within the ECD, human Siglec-15 shares 85% aa sequence identity with mouse and rat Siglec-15. Alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10 (2-5). It is expressed on osteoclasts, macrophages, and dendritic cells (2-6) and binds to the sialyl-Tn antigen (2, 3, 6). This interaction induces the production of TGF-beta by tumor-associated macrophages (3). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (4-6).

• References:

1. Humphrey, M.B. and M.C. Nakamura (2015) Clin. Rev. Allergy Immunol. PMID 26573914.

2. Angata, T. et al. (2007) Glycobiology 17:838.

3. Takamiya, R. et al. (2013) Glycobiology 23:178.

4. Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463.

5. Stuible, M. et al. (2014) J. Biol. Chem. 289:6498.

6. Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.

• Long Name:

  Sialic Acid Binding Ig-like Lectin 15

• Entrez Gene IDs:

  284266 (Human); 620235 (Mouse); 498888 (Rat)

• Alternate Names:

  CD33 antigen-like 3SIGLEC-15; CD33 molecule-like 3; CD33L3; HsT1361; sialic acid binding Ig-like lectin 15; sialic acid-binding Ig-like lectin 15; Siglec15; Siglec-15