• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit IL-21-dependent proliferation of N1186 human T cells Parrish-Novak, J.     et al. (2000) Nature     408:57. The ED    ₅₀ for this effect is 0.6-3.6 μg/mL in the presence of 100 ng/mL of recombinant human IL-21.

• Source

  Human embryonic kidney cell, HEK293-derived Cys20-Pro236, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Cys20

• Predicted Molecular Mass

  26 kDa

• SDS-PAGE

  45-53 kDa, reducing conditions

Data Images

Background: IL-21 R

Interleukin-21 Receptor (IL-21 R )is a type I transmembrane glycoprotein within the class I cytokine receptor family (1). IL-21 R associates with the common gamma  chain ( gamma   _c) which is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15 (2, 3). Mature human IL-21 R consists of a 213 amino acid (aa) extracellular domain (ECD) with 4 conserved cysteine residues, a fibronectin type III domain, and a WSxWS motif, followed by a 21 aa transmembrane domain and a 285 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). Within the ECD, human IL-21 R shares 69% aa identity with mouse and rat IL-21 R, respectively. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 4, 5). Both IL-21 and IL-4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (6). B cell IL-21 R engagement induces Blimp-1 (which mediates plasma cell differentiation) and is important for memory responses (7, 8). IL-21 R engagement enhances NK cell mediated cytotoxic activity and IFN-gamma production (4, 9), control of viral infection and tumor growth by CD8  ⁺ T cells (10), development of regulatory T cells (11), IL-23 responsiveness of encephalitogenic Th17 cells (12), but suppresses the accumulation of IL-17 secreting gamma δ T cells in the airway (13). IL-21 R expression is often upregulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (14, 15).

• References:

1. Tangye, S.G. (2015) Curr. Opin. Immunol. 34:107.

2. Asao, H. et al. (2001) J. Immunol. 167:1.

3. Habib, T. et al. (2002) Biochemistry 41:8725.

4. Parrish-Novak, et al. (2000) Nature 408:57.

5. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.

6. Ozaki, K. et al. (2002) Science 298:1630.

7. Rankin, A.L. et al. (2011) J. Immunol. 186:667.

8. King, I.L. et al. (2010) J. Immunol. 185:6138.

9. Kasaian, M.T. et al. (2002) Immunity 16:559.

10. Frohlich, A. et al. (2009 Science 324:1576.

11. Tortola, L. et al. (2010) Blood 116:5200.

12. Lee, Y. et al. (2015) J. Clin. Invest. 125:4011.

13. Moser, E.K. et al. (2015) PLoS One 10:e0120169.

14. Jin, H. et al. (2009) J. Clin. Invest. 119:47.

15. Sarosiek, K.A. et al. (2010) Blood 115:570.

• Long Name:

  Interleukin 21 Receptor

• Entrez Gene IDs:

  50615 (Human); 60504 (Mouse)

• Alternate Names:

  CD360 antigen; CD360; IL-21 R; IL-21 receptor; IL21R; IL-21R; interleukin 21 receptor; interleukin-21 receptor; MGC10967; NILR; Novel interleukin receptor