• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. The ED    ₅₀ for this effect is 0.4-2.4 μg/mL

• Source

  Human embryonic kidney cell, HEK293-derived

  Human CEACAM-19 (Ala33-Gly157) Accession # Q7Z692-1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Ala33

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  40 kDa

• SDS-PAGE

  42-56 kDa, reducing conditions

Data Images

Background: CEACAM-19

Carcinoembryonic antigen-related cell adhesion molecule-19 (CEACAM-19), also known as CEA-like gene 1 (CEAL1), is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily. Mature human CEACAM-19 consists of a 125 amino acid (aa) extracellular domain, a 21 aa helical transmembrane domain, and a 122 aa cytoplasmic domain (1,2). The extracellular domain contains an Ig-like domain as well as a glycosylation site, while the cytoplasmic domain is predicted to contain at least one immunoreceptor tyrosine-based activation motif (ITAM) (3,4). The extracellular domain of human CEACAM-19 shows 71%, 72%, and 78% aa identity to mouse, rat, and bovine CEACAM-19. The CEACAM family of proteins are involved in numerous intercellular-adhesion and intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (5,6). CEACAM-19 expression has been identified in a wide range of tissues including prostate, uterus, fetal brain, mammary and adrenal glands, skeletal muscle, small intestine, and kidney (1). Human CEACAM-19 has been found to be overexpressed in BT-474, BT20, and T47D breast cancer cell lines as well as LNCaP prostate cancer cells, suggesting a role in tumor progression and metastasis (1,3, 7). The overexpression in breast cancer patients is associated with poor prognosis (7).

• References:

1. Schorilas, A. et al. (2003) Gene 310:79.

2. Estiar, M. et al. (2016) Clin Exp Med doi:10.1007/s10238-016-0442-1.

3. Beauchemin, N. and Arabzadeh, A. (2013) Cancer Metastasis Rev 32 (3-4):643.

4. Kuespert, K. et al. (2006) Curr Opin Cell Biol 18:565.

5. Obrink, B. (1997) Curr Opin Cell Biol 9:616.

6. Horst, AK. and Wagener, C. (2004) Handb Exp Pharmacol 283.

7. Michaelidou, K. et al. (2013) Int J Oncol 42:1770.

• Long Name:

  Carcinoembryonic Antigen-related Cell Adhesion Molecule 19

• Entrez Gene IDs:

  56971 (Human); 319930 (Mouse); 680640 (Rat)

• Alternate Names:

  carcinoembryonic antigen-related cell adhesion molecule 19; CEACAM19; CEACAM-19; CEAL1