• Purity

  >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. When 5 x 10    ⁴ cells/well are added to Recombinant Human DC?SIGN/CD209 Fc Chimera coated plates (5 μg/mL with 100 μL/well), approximately 50-80% of added cells will adhere after 1 hour at room temperature.    
     Optimal dilutions should be determined by each laboratory for each application.  

• Source

  Mouse myeloma cell line, NS0-derived

  MD	Human IgG1 (Pro100-Lys330)	IEGR	Human DC-SIGN (Lys62-Ala404) Accession # Q9NNX6
  N-terminus			C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Met

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  65.7 kDa (monomer)

• SDS-PAGE

  69 kDa, reducing conditions

Background: DC-SIGN/CD209

Human Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 46 kDa, 404 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca  ²⁺-dependent carbohydrate-binding lectin domain (2). Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms (3). DC-SIGN/CD209 is not well conserved between mouse and human, with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2, 4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (5, 6).

• References:

1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.

2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.

3. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.

4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.

5. Geijtenbeek, T.B. et al. (2000) Cell 100:575.

6. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.

• Long Name:

  Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1

• Entrez Gene IDs:

  30835 (Human); 170786 (Mouse)

• Alternate Names:

  CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DCSIGN; DC-SIGN; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1