• Purity

  >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3?E1 mouse preosteoblast cells. The ED    ₅₀ for this effect is 75-450 ng/ml in the presence of 10 ng/ml of Recombinant Mouse Wnt?3a (Catalog # ).

• Source

  Mouse myeloma cell line, NS0-derived Thr25-Arg296, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Thr25

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  31 kDa

• SDS-PAGE

  33-44 kDa, reducing conditions

Data Images

Background: Stanniocalcin 2/STC-2

Mouse STC-2 (Stanniocalcin-2) is a secreted disulfide-linked homodimeric glycoprotein hormone that is related to the STC protein. It is first discovered from corpuscles of stannous, an endocrine gland unique to bony fish (1-3). The 272 amino acid (aa) mature mouse STC-2 has an 86% homology with the human form. STC?2 has been described as having a role in human adult height and is involved in the GIANT consortium (4, 5). STC-2 is expressed in a wide variety of tissues. It regulates calcium and phosphate homeostasis (1-3), enhances mesenchymal stem cell survival (6), promotes cell proliferation in cervical cancer (7), suppresses breast cancer cell migration and invasion (8), promotes osteoblast differentiation (9), and inhibits longitudinal bone growth directly at the growth plate (10). It is also a biomarker for cervical and lung cancers (11, 12).Fform

• References:

1. Wagner, G.F. et al. (1995) Proc. Natl. Acad. Sci. 92:1871.

2. Zhang, J. et al. (1998) Protein Expression and Purification. 12:390.

3. Takei, Y. et al. (2012) Bone. 50:998.

4. Marouli, E. et al. (2017) Nature 542:186.

5. Holmes, D. (2017) Nature Reviews Endocrinology. 13:190.

6. Kim PH, et al. (2015) BMB Rep. 48:702.

7. Wang Y, et al. (2015) Biochem Biophys Res Commun. 466:362.

8. Hou J, et al. (2015) PLoS One. 10:e0122179.

9. Zhou J, et al. (2016) Mol Med Rep. 14:5653.

10. Wu S, et al. (2006) J Biol Chem. 281:5120.

11. Shen XJ, et al. (2014) Int J Clin Exp Pathol. 7: 8770.

12. Na SS, et al. (2015) Biochim Biophys Acta. 1854:668.

• Entrez Gene IDs:

  8614 (Human); 20856 (Mouse)

• Alternate Names:

  Stanniocalcin 2; stanniocalcin-2; STC2; STC-2; STC-2Stanniocalcin-related protein; STC-related protein; STCRP