• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to block anti-TREM-1-induced TNF-alpha secretion by P388D1 mouse lymphoma cells. The ED    ₅₀ for this effect is 2-8 ng/mL in the presence of 0.5-2 μg/mL of Goat Anti-Mouse TREM-1 (Catalog # ).

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse TREM-1 (Ala21-Ser202) Accession # Q9JKE2	SIEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Ala21

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  47 kDa (monomer)

• SDS-PAGE

  60-73 kDa, reducing conditions

Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.

• References:

1. Bouchon, A. (2000) J. Immunol. 164:4991.

2. Bouchon, A. (2001) Nature 410:1103.

3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.

• Long Name:

  Triggering Receptor Expressed on Myeloid Cells 1

• Entrez Gene IDs:

  54210 (Human); 58217 (Mouse)

• Alternate Names:

  CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1