• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.1 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit BDNF-induced proliferation of BaF?TrkB?BD mouse pro?B cells transfected with TrkB. The ED    ₅₀ for this effect is 0.15-0.75 μg/mL in the presence of 16 ng/mL of rhBDNF and 5 μg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # ).

• Source

  Mouse myeloma cell line, NS0-derived Cys32-His429, with a C-terminal 10-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Cys32

• Predicted Molecular Mass

  45.9 kDa

• SDS-PAGE

  75-100 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: TrkB

The neurotrophins, including NGF, BDNF, NT-3 and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). p75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low affinity to transduce cellular signaling pathways that synergize with or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT-3, TrkB binds BDNF, NT-3 and NT-4/5, and TrkC only binds NT-3 (1 - 2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, human and mouse TrkB show 94% amino acid sequence identity (5 - 6). The proteins also exhibit cross-species activity. The primary location of TrkB expression is in the central and peripheral nervous systems. Low level TrkB expression has also been observed in a wide variety of tissues outside the nervous system (6).

• References:

1. Huang, E.J. and L.F. Reichardt (2003) Annu. Rev. Biochem. 72:609.

2. Dechant, G. (2001) Cell Tissue Res. 305:229.

3. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.

4. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.

5. Nakagawara A. et al. (1995) Genomics. 25:538.

6. Klein, R. et al. (1989) EMBO J. 8:3701.

• Long Name:

  Neurotrophic Tyrosine Kinase Receptor B

• Entrez Gene IDs:

  4915 (Human); 18212 (Mouse); 25054 (Rat)

• Alternate Names:

  BDNF/NT-3 growth factors receptor; EC 2.7.10; EC 2.7.10.1; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; NTRK2; TrkB tyrosine kinase; TrkB; trk-B; TRKBBDNF-tropomyosine receptor kinase B; tyrosine kinase receptor B