• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 μL droplet containing 200 ng on a nitrocellulose-coated microplate.    
  All experiments were done under carrier-free conditions.

• Source

  Mouse myeloma cell line, NS0-derived Ile25-Ser420, with a C-terminal 10-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Ile25

• Predicted Molecular Mass

  45.7 kDa

• SDS-PAGE

  100-120 kDa, reducing conditions

Background: OMgp

Oligodendrocyte myelin glycoprotein (OMgp or OMG), Nogo, and myelin-associated glycoprotein (MAG), are three myelin-derived axon outgrowth inhibitors that collapse axonal growth cones and inhibit neurite outgrowth (1 - 3). These three structurally distinct proteins contribute to the myelin-associated inhibitory activity that prevents axonal regeneration after injury of the adult central nervous system (CNS). Mouse OMgp cDNA encodes a 440 amino acid (aa) residue glycosylphosphatidylinositol (GPI)-anchored protein that has a 24 aa signal peptide, eight leucine-rich repeats (LRR) followed by five serine/threonine-rich repeats (4). OMgp has multiple potential N-glycosylation and O-glycosylation sites. Mouse and human OMgp share approximately 88% aa sequence identity. OMgp is expressed on the surface of oligodendrocytes and on large projection neurons, including Purkinje cells of the cerebellum, pyramidal cells of the hippocampus, motoneurons of the brainstem and anterior horn cells of the spinal cord (5). The neurite outgrowth inhibitory activities of all three myelin-derived proteins are mediated by binding to a common receptor complex consisting of the Nogo receptor (NgR) and the p75 neurotrophin receptor (NGFR) (2, 3). Besides its function in the inhibition of axonal growth, OMgp has also been implicated in the inhibition of proliferation. Although the transmembrane receptor that mediates the proliferation inhibition activity has not been identified, the LRR repeats of OMgp were shown to be essential for both the proliferation inhibition and neurite outgrowth inhibition activities (6).

• References:

1. Kottis, V. et al. (2002) J. Neurochem. 82:1566.

2. Wang, K. et al. (2002) Nature 417:941.

3. Wang, K. et al. (2002) Nature 420:74.

4. Mikol, D.D. et al. (1993) Genomics 17:604.

5. Habib, A. et al. (1998) J. Neurochem. 70:1704.

6. Vourc’h, P. et al. (2003) J. Neurochem. 85:889.

• Long Name:

  Oligodendrocyte-Myelin glycoprotein

• Entrez Gene IDs:

  4974 (Human); 18377 (Mouse)

• Alternate Names:

  oligodendrocyte myelin glycoprotein; OMG; OMgp; OMGPoligodendrocyte-myelin glycoprotein