別名:    -Fructose:(acceptor) 5-oxidoreductase

•  Enzyme Commission (EC) Number  1.1.99.11  ( BRENDA | IUBMB )

•  MDL number  MFCD00130905

• Popular Documents:  Specification Sheet (PDF)

產(chǎn)品名稱

General description

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for waste prevention when used in fuel cell research. For more information see the article in biofiles.

包裝

250 units in poly bottle

Unit Definition

One unit will convert 1.0 μmole-fructose to 5-ketofructose per min at pH 4.5 at 37 °C.

Physical form

Lyophilized powder containing citrate-phosphate buffer salts, TRITON? X-100, and stabilizer

Application

D-fructose dehydrogenase is used as a biosensor to detect the presence of D-fructose.

Fructose dehydrogenase (FDH) is used in a number of basic research projects to examine the electrochemical properties of enzyme-catalyzed electrode reactions called bioelectrocatalysis. D-fructose dehydrogenase has been used in a study that contributed towards a convenient method for measuring rare sugars, monosaccharides, for applications in the bio-industry. A direct electron transfer reaction of d-fructose dehydrogenase adsorbed on a porous carbon electrode surface has been used to describe a batch-type coulometric d-fructose biosensor.

Biochem/physiol Actions

D-fructose dehydrogenase catalyzes the oxidation of D-fructose to 5-keto-D-fructose.

Fructose dehydrogenase (FDH) is a heterotrimeric membrane-bound enzyme commonly seen in various Gluconobacter sp. especially in Gluconobacter japonicus (Gluconobacter industrius). It has a molecular mass of ca. 140 kDa, consisting of subunits I (67kDa), II (51 kDa), and III (20 kDa) and catalyzes the oxidation of D-fructose to produce 5-keto-D-fructose. The enzyme is a flavoprotein-cytochrome c complex with subunits I and II covalently bound to flavin adenine dinucleotide (FAD) and heme C as prosthetic groups, respectively.

性質(zhì)