詳細(xì)說明
概述
產(chǎn)品名稱Anti-FXYD1抗體[EPR16082]
描述
兔單克隆抗體[EPR16082] to FXYD1
經(jīng)測試應(yīng)用WB,IP
種屬反應(yīng)性
與反應(yīng): Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human FXYD1 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: O00168陽性對照
WB: Human skeletal muscle, fetal heart and fetal liver lysates; Mouse and rat heart lysates. IP: Human fetal heart whole cell lysate.
常規(guī)說明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲(chǔ)溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA純度Protein A purified
克隆單克隆
克隆編號EPR16082
同種型IgG
研究領(lǐng)域
Cardiovascular
Heart
Contractility
Inotropics
Signal Transduction
Metabolism
Plasma Membrane
Channels
Signal Transduction
Metabolism
Plasma Membrane
ATPases
Anti-FXYD1 antibody [EPR16082] 圖像
Western blot - Anti-FXYD1 antibody [EPR16082] (ab200204)
Anti-FXYD1 antibody [EPR16082] (ab200204) at 1/1000 dilution + Human skeletal muscle lysate at 20 μg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 10 kDa
Observed band size : 12 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-FXYD1 antibody [EPR16082] (ab200204)
All lanes : Anti-FXYD1 antibody [EPR16082] (ab200204) at 1/10000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 20 μg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 10 kDa
Observed band size : 12 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-FXYD1 antibody [EPR16082] (ab200204)
All lanes : Anti-FXYD1 antibody [EPR16082] (ab200204) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate
Lysates/proteins at 10 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 10 kDa
Observed band size : 12 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-FXYD1 antibody [EPR16082] (ab200204)
FXYD1 was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab200204 at 1/120 dilution. Western blot was performed using ab200204 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human fetal heart whole cell lysate 10ug (Input).
Lane 2: ab200204 IP in Human fetal heart whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200204 in Human fetal heart whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.