• Species Reactivity

  Human

• Specificity

  Detects human CCL7/MCP-3/MARC in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) CCL2/MCP-1, rhCCL8/MCP?2, or rhCCL13/MCP?4 is observed.

• Source

  Monoclonal Mouse IgG1 Clone # 36320

• Immunogen

  E. coli-derived recombinant human CCL7/MCP-3/MARC    
  Gln34-109    
  Accession # Q7Z7Q8

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 647

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  THP?1 human acute monocytic leukemia cell line fixed with paraformaldehyde and permeabilized with saponin

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL7/MCP-3/MARC

MCP-2 and CCL7 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and CCL7 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. CCL7 also shares 58% amino acid identity with MCP-2.

CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites.

Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4⁺ and CD8⁺ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

• Entrez Gene IDs:

  6354 (Human); 20306 (Mouse)

• Alternate Names:

  C-C motif chemokine 7; CCL7; chemokine (C-C motif) ligand 7; FIC; MARC; MCP-3; MCP3Monocyte chemotactic protein 3; MCP-3Small-inducible cytokine A7; MGC138465; Monocyte chemoattractant protein 3; NC28SCYA6; SCYA7MGC138463; small inducible cytokine A7 (monocyte chemotactic protein 3)