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Anti-Pellino 1 + Pellino 2 antibody [EPR19282] 100μl

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Anti-Pellino 1 + Pellino 2 antibody [EPR19282] 100μl信息二維碼

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產(chǎn)品介紹

    基本參數(shù)

    詳細說明

    概述

    • 產(chǎn)品名稱Anti-Pellino 1 + Pellino 2抗體[EPR19282]

    • 描述

      兔單克隆抗體[EPR19282] to Pellino 1 + Pellino 2

    • 經(jīng)測試應(yīng)用WB

    • 種屬反應(yīng)性

      與反應(yīng): Mouse, Rat, Human    
       

    • 免疫原

      Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Pellino 1 aa 150-250. The exact sequence is proprietary.
      Database link: Q96FA3

    • 陽性對照    

      • WB: Human Pellino 1 and Pellino 2 full length recombinant proteins; Ramos, PC-12, Jurkat, Daudi, HeLa, C6 and NIH/3T3 whole cell lysates; RAW 264.7 treated with 1 μg/ml LPS for 6 hours whole cell lysate; Mouse and Rat brain lysates.

    • 常規(guī)說明

      This product is a recombinant rabbit monoclonal antibody.

      Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    性能

    • 形式Liquid

    • 存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    • 存儲溶液Preservative: 0.01% Sodium azide
      Constituents: 59% PBS, 40% Glycerol, 0.05% BSA


    • 純度Protein A purified

    • 克隆單克隆

    • 克隆編號EPR19282

    • 同種型IgG

    • 研究領(lǐng)域

      • Epigenetics and Nuclear Signaling

      • Cell cycle

      • Kinases/Phosphatases

      • Other

      • Signal Transduction

      • Signaling Pathway

      • Nuclear Signaling

      • NFkB Pathway

      • Signal Transduction

      • Protein Phosphorylation

      • Ser / Thr Kinases

      • Other Kinases

      • Signal Transduction

      • Protein Phosphorylation

      • Ser / Thr Kinases

      • MAPK Pathway

      Anti-Pellino 1 + Pellino 2 antibody [EPR19282] 圖像

      • Western blot - Anti-Pellino 1 antibody [EPR19282] (ab192833)

        Western blot - Anti-Pellino 1 antibody [EPR19282] (ab192833)          

           All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/5000 dilution

        Lane 1 : Human Pellino 1 full length recombinant protein
        Lane 2 : Human Pellino 2 full length recombinant protein
        Lane 3 : Human Pellino 3 full length recombinant protein

        Lysates/proteins at 0.01 μg per lane.

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size : 46 kDa
        Observed band size : 46 kDa
           

        Blocking/dilution buffer: 5% NFDM/TBST.

        Exposure times: Lane 1:1 second; Lane 2 and 3:1 minute.

        Human Pellino 1 full length recombinant protein contains aa1-418 with a His-Tag?. Human Pellino 2 full length recombinant protein contains aa1-420 with a His-Tag?. Human Pellino 3  full length recombinant protein contains aa1-469 with a His-Tag?. All three recombinant proteins were made in-house.

         

      • Western blot - Anti-Pellino 1 antibody [EPR19282] (ab192833)

        Western blot - Anti-Pellino 1 antibody [EPR19282] (ab192833)          

           All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution

        Lane 1 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
        Lane 2 : Untreated RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
        Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 1 μg/ml LPS for 6 hours, whole cell lysate
        Lane 4 : Untreated PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
        Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) treated with 1 μg/ml LPS for 6 hours whole cell lysate

        Lysates/proteins at 20 μg per lane.

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size : 46 kDa
        Observed band size : 46 kDa


        Exposure time : 3 minutes    

        Blocking/dilution buffer: 5% NFDM/TBST.

        The expression of Pellino 1 can be induced by LPS. (PMID: 21204785).

         

      • Western blot - Anti-Pellino 1 antibody [EPR19282] (ab192833)

        Western blot - Anti-Pellino 1 antibody [EPR19282] (ab192833)          

           All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution

        Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
        Lane 2 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate
        Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

        Lysates/proteins at 10 μg per lane.

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size : 46 kDa
        Observed band size : 46 kDa


        Exposure time : 3 minutes    

        Blocking/dilution buffer: 5% NFDM/TBST.

         

      • Western blot - Anti-Pellino 1 antibody [EPR19282] (ab192833)

        Western blot - Anti-Pellino 1 antibody [EPR19282] (ab192833)          

           All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution

        Lane 1 : Mouse brain lysate
        Lane 2 : Rat brain lysate
        Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
        Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

        Lysates/proteins at 10 μg per lane.

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size : 46 kDa
        Observed band size : 46 kDa


        Exposure time : 3 minutes    

        Blocking/dilution buffer: 5% NFDM/TBST.

         

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