• Species Reactivity

  Mouse

• Specificity

  Detects mouse FGF-15 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) FGF-19 is observed and less than 5% cross-reactivity with rhFGF-22 and rhFGF-23 is observed.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant mouse FGF-15    
  Ser24-Lys218    
  Accession # O35622

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunohistochemistry

  5-15 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.2 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-15

FGF-15 (Fibroblast growth factor 15) is a 25 kDa, secreted, nonglycosylated polypeptide that is a member of the FGF-15/21/23 subfamily, FGF family of growth factors. It is synthesized by ileal epithelium, colonic myofibroblasts, and select areas of the developing brain. FGF-15 acts on hepatocytes and reduces CYP7A1 levels, thus blocking bile acid production. It also promotes colonic epithelial migration, and in the embryonic brain, inhibits over-production of neuronal precursors. FGF-15 is synthesized as a 218 amino acid (aa) precursor that contains a 25 aa signal sequence and a 182 aa mature region (aa 26-218; 23 kDa predicted MW). Mature FGF-15 contains a characteristic 3-dimensional beta -trefoil structure, plus a unique Cys pattern that contributes to stability. There are two potential splice forms. One shows a 23 aa substitution for aa 120-218, while a second shows a 41 aa substitution for aa 120-218. Over aa 23-200, mouse FGF-15 shares 96% aa identity with rat FGF-15. Although mouse FGF-15 is considered the ortholog to human FGF-19, the two molecules share only 54% aa identity over aa 23-200, and are produced by different cell types (intestinal epithelium in mouse, hepatocytes in human).

• Long Name:

  Fibroblast Growth Factor 15

• Entrez Gene IDs:

  14170 (Mouse)

• Alternate Names:

  FGF15; FGF-15; FGF-19