• Species Reactivity

  Human

• Specificity

  Detects human Notch-1 Intracellular Domain in Western blots. In Western blots, this antibody does not cross-react with the corresponding intracellular segments of recombinant human (rh) Notch-2, rhNotch-3, or rhNotch-4.

• Source

  Monoclonal Mouse IgG    _2A Clone # 433802

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Notch?1    
  Gly2428-Lys2556    
  Accession # AAG33848

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  Recombinant Human Notch?1 Intracellular Domain

• 
  

• Immunocytochemistry

  8-25 μg/mL

  Immersion fixed Saos-2 human osteosarcoma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Notch-1

Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes (1-3). Notch signaling is important for maintaining stem cells and inducing differentiation, especially in the nervous system and lymphoid tissues (2-4). Notch can specify binary cell fates; for example, promoting T- over B-cell development from a common precursor (2). More than 50% of human T-lineage acute lymphoblastic leukemia (T?ALL) have activating mutations of Notch1 (1, 5). Human Notch-1 is synthesized as a 2556 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1718 aa extracellular domain (ECD) with 36 EGF-like repeats and three Lin-12/Notch repeats (LNR), a 23 aa transmembrane (TM) segment and a 785 aa cytoplasmic domain containing six ankyrin repeats, a glutamine-rich domain and a PEST sequence. The 11^th and 12^th EGF-like repeats bind ligands including Jagged and Delta-like families in humans (6). O-fucosylation by Fringe family members at a site within this region can inhibit the interaction of Notch with Jagged ligands, thereby promoting Delta-like ligand interactions (7). Notch-1 receptor undergoes post-translational furin-type proteolytic cleavage, forming a heterodimer through interaction of a hydrophobic area C-terminal to the LNR on the 1647 aa ligand-binding extracellular region with the 891 aa transmembrane/cytoplasmic portion (8, 9). Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme (ADAM17) and the presenilin-dependent gamma -secretase results in the release of the Notch intracellular domain (NICD) which translocates into the nucleus, activating transcription of Notch-responsive genes (10). Human Notch-1 ECD aa 19-526, including the first 13 EGF repeats, shows 91% aa identity with corresponding regions of mouse and rat, 89% with canine, and 79% with chicken Notch-1. This region also exhibits 60% aa identity with human Notch-2 and Notch-3.

• References:

1. Ellisen, L.W. et al. (1991) Cell 66:649.

2. Dumortier, A. et al. (2005) Int. J. Hematol. 82:277.

3. Yoon, K. and N. Gaiano (2005) Nat. Neurosci. 8:709.

4. Androutsellis-Theotokis, A. et al. (2006) Nature 442:823.

5. Weng, A.P. et al. (2004) Science 306:269.

6. Hambleton, S. et al. (2004) Structure 12:2173.

7. Yang, L. et al. (2005) Mol. Biol. Cell 16:927.

8. Sanchez-Irizarry, C. et al. (2004) Mol. Cell. Biol. 24:9265.

9. Logeat, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8108.

10. Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.

• Entrez Gene IDs:

  4851 (Human); 25496 (Rat)

• Alternate Names:

  EC 2.1.2.11; EC 3.4.21.68; hN1; Notch (Drosophila) homolog 1 (translocation-associated); notch 1Notch homolog 1, translocation-associated (Drosophila); Notch homolog 1, translocation-associated; Notch1; Notch-1; TAN1; TAN1neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1